Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23513 | 5' | -58.5 | NC_005259.1 | + | 24053 | 0.68 | 0.526631 |
Target: 5'- gUCGUCggcgCGGUGCUCAaucucgugCACCGCGA-GAu -3' miRNA: 3'- -GGCAG----GCCGCGAGUa-------GUGGCGCUgCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 18326 | 0.68 | 0.516638 |
Target: 5'- aCCGaggCCGGUGCguUCGUggucgaCGCCGaCGGCGAc -3' miRNA: 3'- -GGCa--GGCCGCG--AGUA------GUGGC-GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 51113 | 0.68 | 0.516638 |
Target: 5'- uCUGUCCGGCagGCcaUCAgaUCACCGUcggGGCGGc -3' miRNA: 3'- -GGCAGGCCG--CG--AGU--AGUGGCG---CUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 28751 | 0.69 | 0.496889 |
Target: 5'- gCGcCCGaGCGUgagaUCGUCGCCGgugcCGACGAg -3' miRNA: 3'- gGCaGGC-CGCG----AGUAGUGGC----GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 4331 | 0.69 | 0.487142 |
Target: 5'- cUCGUgCCGGaC-CUCGUCGCgGUGAUGAg -3' miRNA: 3'- -GGCA-GGCC-GcGAGUAGUGgCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 19078 | 0.7 | 0.430715 |
Target: 5'- gCgGUCCGGCGCUacgacaaGCUGCG-CGAa -3' miRNA: 3'- -GgCAGGCCGCGAguag---UGGCGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 7404 | 0.71 | 0.38674 |
Target: 5'- cCCGcaaCGGCaucCUCGUCGCCGuCGGCGGu -3' miRNA: 3'- -GGCag-GCCGc--GAGUAGUGGC-GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 752 | 0.71 | 0.353758 |
Target: 5'- gCCG-CCGGUGCcuaccgCGUcCACCGCGcCGAg -3' miRNA: 3'- -GGCaGGCCGCGa-----GUA-GUGGCGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 13454 | 0.72 | 0.307393 |
Target: 5'- aCCGUCggcgCGGCGCUgGugcUCACCGCgcugcccGACGAu -3' miRNA: 3'- -GGCAG----GCCGCGAgU---AGUGGCG-------CUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 58970 | 0.73 | 0.293933 |
Target: 5'- -gGUCgGGCaGCUCGUCGgCGaCGGCGAg -3' miRNA: 3'- ggCAGgCCG-CGAGUAGUgGC-GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 56077 | 0.74 | 0.248315 |
Target: 5'- aCCGUCgagcagauCGGUGCUCGUCggggccACCGCGugGc -3' miRNA: 3'- -GGCAG--------GCCGCGAGUAG------UGGCGCugCu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 61746 | 0.75 | 0.208711 |
Target: 5'- gCGUCCGGCGag-AUCugUGCGGCGGc -3' miRNA: 3'- gGCAGGCCGCgagUAGugGCGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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