Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23514 | 5' | -57.8 | NC_005259.1 | + | 58983 | 0.7 | 0.428295 |
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Target: 5'- gUCGgCGACGGcgagCUUGGCCuuuucacCGUUGAg -3' miRNA: 3'- gAGCgGCUGCCa---GAACCGGu------GCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 26506 | 0.7 | 0.450612 |
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Target: 5'- gCUCGCCGcccggcgagaagagcGCGGUgagcagCUUGGCCACcaCGAu -3' miRNA: 3'- -GAGCGGC---------------UGCCA------GAACCGGUGcaGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 49748 | 0.69 | 0.485241 |
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Target: 5'- gCUCGUCaguGACGGUCUU-GCCgucuGCGUUGAa -3' miRNA: 3'- -GAGCGG---CUGCCAGAAcCGG----UGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 52608 | 0.68 | 0.555855 |
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Target: 5'- -gCGCCGuACGGU---GGCCaacuACGUCGAg -3' miRNA: 3'- gaGCGGC-UGCCAgaaCCGG----UGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 20068 | 0.68 | 0.566214 |
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Target: 5'- -gCGgUGGCGGUCgucaCCACGUCGAa -3' miRNA: 3'- gaGCgGCUGCCAGaaccGGUGCAGCU- -5' |
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| 23514 | 5' | -57.8 | NC_005259.1 | + | 38064 | 0.66 | 0.681709 |
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Target: 5'- -cUGCCGACGaGccgccaUCgagcaUGGcCCACGUCGAg -3' miRNA: 3'- gaGCGGCUGC-C------AGa----ACC-GGUGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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