Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2353 | 3' | -60.3 | NC_001416.1 | + | 17402 | 1.05 | 0.000481 |
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Target: 5'- gCUGCACCGCUGGCGGCGUGACACCAUu -3' miRNA: 3'- -GACGUGGCGACCGCCGCACUGUGGUA- -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 44941 | 0.71 | 0.17867 |
|
Target: 5'- gUGCugUuGCUGgGCGGCGaUGACGCCu- -3' miRNA: 3'- gACGugG-CGAC-CGCCGC-ACUGUGGua -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 5666 | 0.7 | 0.220794 |
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Target: 5'- -gGCACCGCUGGCugcagguaacccGGCaucUGAUGCCGUu -3' miRNA: 3'- gaCGUGGCGACCG------------CCGc--ACUGUGGUA- -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 14230 | 0.69 | 0.244874 |
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Target: 5'- -cGCAcgacuCCGCUGGCgcacaucgccgGGCGUG-CGCCAg -3' miRNA: 3'- gaCGU-----GGCGACCG-----------CCGCACuGUGGUa -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 5490 | 0.69 | 0.228984 |
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Target: 5'- gCUGCGCCGCgcUGGCGuucucgcccuccgucGC-UGGCACCAc -3' miRNA: 3'- -GACGUGGCG--ACCGC---------------CGcACUGUGGUa -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 2474 | 0.67 | 0.322328 |
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Target: 5'- aCUGCuucguuuaugCGCUGGCGGCGcUGcGCAUCAg -3' miRNA: 3'- -GACGug--------GCGACCGCCGC-AC-UGUGGUa -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 18362 | 0.66 | 0.407422 |
|
Target: 5'- uUGcCGCCGCUGGUaauGGUG-GGgGCCGUc -3' miRNA: 3'- gAC-GUGGCGACCG---CCGCaCUgUGGUA- -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 11331 | 0.66 | 0.371734 |
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Target: 5'- -gGCAUUGCaGGCGGCGaacGAgGCCGc -3' miRNA: 3'- gaCGUGGCGaCCGCCGCa--CUgUGGUa -5' |
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| 2353 | 3' | -60.3 | NC_001416.1 | + | 16956 | 0.66 | 0.388418 |
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Target: 5'- -aGCACCGgUGGCgugugcuGGCGgUGAacaGCCAg -3' miRNA: 3'- gaCGUGGCgACCG-------CCGC-ACUg--UGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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