Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23534 | 3' | -48.6 | NC_005260.1 | + | 43965 | 0.66 | 0.999865 |
Target: 5'- -aGUUGCUCACAggGUGGUguacGCGgUGGCg -3' miRNA: 3'- acUAGUGAGUGU--UAUCGu---CGCaACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 135445 | 0.66 | 0.999865 |
Target: 5'- -uGUCAgUCACAAgAGaCGGCGcgGGUg -3' miRNA: 3'- acUAGUgAGUGUUaUC-GUCGCaaCCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 155982 | 0.66 | 0.999854 |
Target: 5'- gGAUUGCgCACca-GGCAGCGUggaaacuucgguagUGGCu -3' miRNA: 3'- aCUAGUGaGUGuuaUCGUCGCA--------------ACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 186532 | 0.66 | 0.999826 |
Target: 5'- --uUCACUCuCAGUAagAGCGaUGGCa -3' miRNA: 3'- acuAGUGAGuGUUAUcgUCGCaACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 25467 | 0.66 | 0.999778 |
Target: 5'- ----aGCg-ACGAUGGCcGCGUUGGCc -3' miRNA: 3'- acuagUGagUGUUAUCGuCGCAACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 58657 | 0.66 | 0.999718 |
Target: 5'- aUGAUCACUUACGgcaucAUGGCcAGCaaUUGGa -3' miRNA: 3'- -ACUAGUGAGUGU-----UAUCG-UCGc-AACCg -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 164269 | 0.67 | 0.999644 |
Target: 5'- uUGA--GCUUugGuuuuguUGGCAGCGUuaaUGGCa -3' miRNA: 3'- -ACUagUGAGugUu-----AUCGUCGCA---ACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 197112 | 0.67 | 0.999644 |
Target: 5'- ----uGC-CACAgcuAUGGCcGCGUUGGCa -3' miRNA: 3'- acuagUGaGUGU---UAUCGuCGCAACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 53051 | 0.67 | 0.999636 |
Target: 5'- ---gCACUCGCAAUGGCAaacacccaaucaaGCcguucgauuauGUUGGCc -3' miRNA: 3'- acuaGUGAGUGUUAUCGU-------------CG-----------CAACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 5463 | 0.67 | 0.999554 |
Target: 5'- aGAUCugcgauUUCGCAGU-GCAGCGgcucagGGUg -3' miRNA: 3'- aCUAGu-----GAGUGUUAuCGUCGCaa----CCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 114286 | 0.67 | 0.999445 |
Target: 5'- cUGGUCAUUCugAAUGauucCAGCGUUGu- -3' miRNA: 3'- -ACUAGUGAGugUUAUc---GUCGCAACcg -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 193890 | 0.67 | 0.999157 |
Target: 5'- aUGAuUCGCgUCGCAAUG--AGCGUUGcGCa -3' miRNA: 3'- -ACU-AGUG-AGUGUUAUcgUCGCAAC-CG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 219507 | 0.68 | 0.99897 |
Target: 5'- aGAaCACUCACAAccggAGCcGUGaUUGGUg -3' miRNA: 3'- aCUaGUGAGUGUUa---UCGuCGC-AACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 114533 | 0.68 | 0.998748 |
Target: 5'- gGAUCgaACUCAC-AUGGC-GCGUUaGCa -3' miRNA: 3'- aCUAG--UGAGUGuUAUCGuCGCAAcCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 63894 | 0.68 | 0.998182 |
Target: 5'- cUGGUgACUCaaGCAAUGGCAGg--UGGUa -3' miRNA: 3'- -ACUAgUGAG--UGUUAUCGUCgcaACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 34748 | 0.68 | 0.998182 |
Target: 5'- gGAUCACguuaGCAGaAGCaagAGCG-UGGCa -3' miRNA: 3'- aCUAGUGag--UGUUaUCG---UCGCaACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 132760 | 0.68 | 0.997788 |
Target: 5'- cUGcUCugUUAUcaugugaAAUccAGCAGCGUUGGCc -3' miRNA: 3'- -ACuAGugAGUG-------UUA--UCGUCGCAACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 49259 | 0.68 | 0.997709 |
Target: 5'- aGAUCGCggauaCACAAUcaccGGCAGCGgUaugaacucucgcuaUGGCa -3' miRNA: 3'- aCUAGUGa----GUGUUA----UCGUCGC-A--------------ACCG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 165860 | 0.69 | 0.997414 |
Target: 5'- cGGUCACUCGCuugcgAGguGCGguaucUGcGCu -3' miRNA: 3'- aCUAGUGAGUGuua--UCguCGCa----AC-CG- -5' |
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23534 | 3' | -48.6 | NC_005260.1 | + | 77379 | 0.69 | 0.996938 |
Target: 5'- cGGUCAgaUUCGCG--GGCAGCcaUGGCu -3' miRNA: 3'- aCUAGU--GAGUGUuaUCGUCGcaACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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