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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23583 | 5' | -50.8 | NC_005261.1 | + | 109677 | 0.68 | 0.975312 |
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Target: 5'- gGUGCUGAGcggACGAGGGacGAUGgccgcGCCUa -3' miRNA: 3'- -CGCGACUUca-UGCUCCU--CUACa----UGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 111943 | 1.11 | 0.006271 |
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Target: 5'- gGCGCUGAAGUACGAGGAGAUGUACCUg -3' miRNA: 3'- -CGCGACUUCAUGCUCCUCUACAUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 112671 | 0.69 | 0.955423 |
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Target: 5'- aGCgGCUGGAGcGCGAGGGGcUGggcgucgagGCCg -3' miRNA: 3'- -CG-CGACUUCaUGCUCCUCuACa--------UGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 115054 | 0.67 | 0.977859 |
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Target: 5'- gGCGaaGggGgGCGGGGuggGGAUGUGCUUc -3' miRNA: 3'- -CGCgaCuuCaUGCUCC---UCUACAUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 115322 | 0.68 | 0.966397 |
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Target: 5'- uGCGCgaucggGggGUACcGGGAcGUGUACUUc -3' miRNA: 3'- -CGCGa-----CuuCAUGcUCCUcUACAUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 115991 | 0.74 | 0.778416 |
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Target: 5'- gGCGCgGAGGgGCGGGGcGGggGUGCCg -3' miRNA: 3'- -CGCGaCUUCaUGCUCC-UCuaCAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 126066 | 0.68 | 0.962977 |
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Target: 5'- gGCGCUGGccuGUGCGGGGcu-UGggGCCUg -3' miRNA: 3'- -CGCGACUu--CAUGCUCCucuACa-UGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 131761 | 0.68 | 0.972558 |
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Target: 5'- gGCGcCUGggGUG-GGGcGGGGUGUGCg- -3' miRNA: 3'- -CGC-GACuuCAUgCUC-CUCUACAUGga -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 132667 | 0.67 | 0.98614 |
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Target: 5'- cGCGUguGGGUGCGAGGAcaaGAUGgGCUUc -3' miRNA: 3'- -CGCGacUUCAUGCUCCU---CUACaUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 136597 | 0.66 | 0.992889 |
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Target: 5'- gGCGCUGggGcuCGGGGAGccGgACUUc -3' miRNA: 3'- -CGCGACuuCauGCUCCUCuaCaUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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