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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23583 | 5' | -50.8 | NC_005261.1 | + | 13280 | 0.66 | 0.989264 |
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Target: 5'- aCGCUGuuGUugGCGGGGcgcAGGUGUGCg- -3' miRNA: 3'- cGCGACuuCA--UGCUCC---UCUACAUGga -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 94727 | 0.66 | 0.989264 |
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Target: 5'- gGCGCUGgcGcaGCGcAGGAGGUccGCCg -3' miRNA: 3'- -CGCGACuuCa-UGC-UCCUCUAcaUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 86927 | 0.66 | 0.99181 |
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Target: 5'- gGCGCcGgcGUgcGCGAGGuAGGUGgccgcgGCCa -3' miRNA: 3'- -CGCGaCuuCA--UGCUCC-UCUACa-----UGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 45028 | 0.66 | 0.992889 |
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Target: 5'- cGCuGCUGGAGccCGAGGcuGUGUACa- -3' miRNA: 3'- -CG-CGACUUCauGCUCCucUACAUGga -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 98655 | 0.66 | 0.992889 |
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Target: 5'- gGCGCUGggGagggaGAGGGGGacagACCg -3' miRNA: 3'- -CGCGACuuCaug--CUCCUCUaca-UGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 37606 | 0.66 | 0.992889 |
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Target: 5'- cGCGCgccuGUGCGAGcGGGAccUGcACCUg -3' miRNA: 3'- -CGCGacuuCAUGCUC-CUCU--ACaUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 39240 | 0.66 | 0.992889 |
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Target: 5'- aCGCUGAAGauugcCGAGGAcGAcGUGCUc -3' miRNA: 3'- cGCGACUUCau---GCUCCU-CUaCAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 76041 | 0.66 | 0.992889 |
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Target: 5'- cGCGCUGcacauGGUcuucaacgucaACGAGGGGA---GCCUg -3' miRNA: 3'- -CGCGACu----UCA-----------UGCUCCUCUacaUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 32079 | 0.66 | 0.992889 |
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Target: 5'- gGCGCUGggGcuCGGGGAGccGgACUUc -3' miRNA: 3'- -CGCGACuuCauGCUCCUCuaCaUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 136597 | 0.66 | 0.992889 |
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Target: 5'- gGCGCUGggGcuCGGGGAGccGgACUUc -3' miRNA: 3'- -CGCGACuuCauGCUCCUCuaCaUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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