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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23583 | 5' | -50.8 | NC_005261.1 | + | 76173 | 0.7 | 0.937337 |
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Target: 5'- gGCGCUGcAGUACGAGcGGGGccugggcGUGCUg -3' miRNA: 3'- -CGCGACuUCAUGCUC-CUCUa------CAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 58295 | 0.7 | 0.915137 |
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Target: 5'- cGCGCcGggGggcGCGGGGAGggGgcgGCCc -3' miRNA: 3'- -CGCGaCuuCa--UGCUCCUCuaCa--UGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 14689 | 0.71 | 0.895782 |
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Target: 5'- uCGCUGAuGGUgGCGAGGGGggGcGCCUu -3' miRNA: 3'- cGCGACU-UCA-UGCUCCUCuaCaUGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 13832 | 0.72 | 0.842128 |
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Target: 5'- gGCGCUGcAGUACcggcuGGGGGUGggggGCCUg -3' miRNA: 3'- -CGCGACuUCAUGcu---CCUCUACa---UGGA- -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 115991 | 0.74 | 0.778416 |
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Target: 5'- gGCGCgGAGGgGCGGGGcGGggGUGCCg -3' miRNA: 3'- -CGCGaCUUCaUGCUCC-UCuaCAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 11474 | 0.74 | 0.778416 |
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Target: 5'- gGCGCgGAGGgGCGGGGcGGggGUGCCg -3' miRNA: 3'- -CGCGaCUUCaUGCUCC-UCuaCAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 79088 | 0.74 | 0.748783 |
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Target: 5'- aGCGCcuucaUGggGUugcugcggagGCG-GGAGAUGUACCg -3' miRNA: 3'- -CGCG-----ACuuCA----------UGCuCCUCUACAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 55297 | 0.76 | 0.665631 |
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Target: 5'- gGCGCUGAagagccGGUGCGGGGGGucaggggGUGCUg -3' miRNA: 3'- -CGCGACU------UCAUGCUCCUCua-----CAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 51909 | 0.77 | 0.618759 |
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Target: 5'- cGCGCUGAAGUACGuggccagcacgcuGGAGGgcgacGUGCCc -3' miRNA: 3'- -CGCGACUUCAUGCu------------CCUCUa----CAUGGa -5' |
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| 23583 | 5' | -50.8 | NC_005261.1 | + | 111943 | 1.11 | 0.006271 |
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Target: 5'- gGCGCUGAAGUACGAGGAGAUGUACCUg -3' miRNA: 3'- -CGCGACUUCAUGCUCCUCUACAUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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