Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23648 | 3' | -53.8 | NC_005261.1 | + | 129715 | 0.66 | 0.952009 |
Target: 5'- -aGGCAGGcCCGCcGGCUGcaGCGg-- -3' miRNA: 3'- ggUCGUCCaGGUGuUCGACa-CGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 62933 | 0.66 | 0.952009 |
Target: 5'- aCCGGCAGGcagccCCGCAccGGCgagGCGUc- -3' miRNA: 3'- -GGUCGUCCa----GGUGU--UCGacaCGCAac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 109873 | 0.66 | 0.947724 |
Target: 5'- gCAGCGGGgcCCGCGGGCgg-GCGc-- -3' miRNA: 3'- gGUCGUCCa-GGUGUUCGacaCGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 89265 | 0.66 | 0.943193 |
Target: 5'- gUguGCGGG-CgACGGGCUG-GCGUUc -3' miRNA: 3'- -GguCGUCCaGgUGUUCGACaCGCAAc -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 50829 | 0.66 | 0.938414 |
Target: 5'- gCGGCuuGGcgCCGCGAGCUG-GCGccugUGg -3' miRNA: 3'- gGUCGu-CCa-GGUGUUCGACaCGCa---AC- -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 2209 | 0.66 | 0.938414 |
Target: 5'- cCCAGCg---CCGCGAGCagGUGCGa-- -3' miRNA: 3'- -GGUCGuccaGGUGUUCGa-CACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 85943 | 0.66 | 0.933384 |
Target: 5'- gCGGcCAGGgcgCCGCGgaAGCUG-GCGUg- -3' miRNA: 3'- gGUC-GUCCa--GGUGU--UCGACaCGCAac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 123506 | 0.66 | 0.933384 |
Target: 5'- aCCAGCGGcagugccgcGUCCgcuaGCAAGCUGUaccgcgcgaGCGUg- -3' miRNA: 3'- -GGUCGUC---------CAGG----UGUUCGACA---------CGCAac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 19052 | 0.67 | 0.928103 |
Target: 5'- gCCAgGCAGGggUACGGGCUGcGCGg-- -3' miRNA: 3'- -GGU-CGUCCagGUGUUCGACaCGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 63351 | 0.67 | 0.928103 |
Target: 5'- cCCA-CGGGUUCACGguGGCgcgGUGCGg-- -3' miRNA: 3'- -GGUcGUCCAGGUGU--UCGa--CACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 98153 | 0.67 | 0.928103 |
Target: 5'- gCGGCaAGG-CCGCGGGCggcggGUGUGUc- -3' miRNA: 3'- gGUCG-UCCaGGUGUUCGa----CACGCAac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 87679 | 0.67 | 0.923134 |
Target: 5'- gCCAGCGGuGagucgcagcuccgcgCCGCAAGCaGUGCGa-- -3' miRNA: 3'- -GGUCGUC-Ca--------------GGUGUUCGaCACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 129381 | 0.67 | 0.92257 |
Target: 5'- gCGGCuucGGGUCCAcCGGGCUGUGa---- -3' miRNA: 3'- gGUCG---UCCAGGU-GUUCGACACgcaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 78236 | 0.67 | 0.916785 |
Target: 5'- cUCAGCAG--CCGCAGGC-GUGCGa-- -3' miRNA: 3'- -GGUCGUCcaGGUGUUCGaCACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 98911 | 0.67 | 0.916785 |
Target: 5'- aCAGCGGGUCgGCGGGgucgGcUGCGggGg -3' miRNA: 3'- gGUCGUCCAGgUGUUCga--C-ACGCaaC- -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 117466 | 0.67 | 0.916785 |
Target: 5'- gCGGCAGGUCgGCGGcgacgcGCUGcUGCGc-- -3' miRNA: 3'- gGUCGUCCAGgUGUU------CGAC-ACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 104805 | 0.67 | 0.910748 |
Target: 5'- uCCAGCGGGUCCcGCuccgcAGCg--GCGUc- -3' miRNA: 3'- -GGUCGUCCAGG-UGu----UCGacaCGCAac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 11742 | 0.67 | 0.910748 |
Target: 5'- uCCAGCAGa--CGCGAGC-GUGCGa-- -3' miRNA: 3'- -GGUCGUCcagGUGUUCGaCACGCaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 14785 | 0.67 | 0.910131 |
Target: 5'- cCCGcGCAGGUgccgcucCCGCAGGCUGcGCa--- -3' miRNA: 3'- -GGU-CGUCCA-------GGUGUUCGACaCGcaac -5' |
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23648 | 3' | -53.8 | NC_005261.1 | + | 50300 | 0.67 | 0.903821 |
Target: 5'- cCCAGCAGGUCCucguccacucgguGCAAGCUuuccaccUGCa--- -3' miRNA: 3'- -GGUCGUCCAGG-------------UGUUCGAc------ACGcaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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