Results 1 - 20 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23740 | 5' | -56.3 | NC_005261.1 | + | 118073 | 0.66 | 0.864267 |
Target: 5'- --aGCGgccGCAgcCGCGCUCGUcugcuggCGGCCCGc -3' miRNA: 3'- aagUGCa--UGU--GCGCGAGCA-------GCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 30249 | 0.66 | 0.8866 |
Target: 5'- -gCGCgGUACucgACGCGCggcgCGgCGGCCCGc -3' miRNA: 3'- aaGUG-CAUG---UGCGCGa---GCaGCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 38283 | 0.66 | 0.8866 |
Target: 5'- -gCugGUGCACGCGg-UGcUGGCCCGc -3' miRNA: 3'- aaGugCAUGUGCGCgaGCaGCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 1827 | 0.66 | 0.89335 |
Target: 5'- -gCGCGUaagcggccucgGCGCGCGCgaaggCGcCGgGCCCGa -3' miRNA: 3'- aaGUGCA-----------UGUGCGCGa----GCaGC-UGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 39894 | 0.66 | 0.878203 |
Target: 5'- -gCGCGgcugugcagcugGCGCGCGCggcCGUCGccACCCa -3' miRNA: 3'- aaGUGCa-----------UGUGCGCGa--GCAGC--UGGGc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 49226 | 0.66 | 0.89335 |
Target: 5'- --gGCGcccaGCGCGCGCagGUCGGCCg- -3' miRNA: 3'- aagUGCa---UGUGCGCGagCAGCUGGgc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 107649 | 0.66 | 0.879624 |
Target: 5'- -cCGCGcUGCGCGCGCaaacucCG-CGGCCCc -3' miRNA: 3'- aaGUGC-AUGUGCGCGa-----GCaGCUGGGc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 11890 | 0.66 | 0.89335 |
Target: 5'- -cCGCccGCACGCGCgcccgccgCGggccCGGCCCGg -3' miRNA: 3'- aaGUGcaUGUGCGCGa-------GCa---GCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 12315 | 0.66 | 0.865019 |
Target: 5'- -gCGCGUagccggcggGCGCGCGCUUGgcCGGCgCGg -3' miRNA: 3'- aaGUGCA---------UGUGCGCGAGCa-GCUGgGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 131480 | 0.66 | 0.865019 |
Target: 5'- -cCGCGa--GCGCGC-CGUCGACgCCu -3' miRNA: 3'- aaGUGCaugUGCGCGaGCAGCUG-GGc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 106978 | 0.66 | 0.879624 |
Target: 5'- -gCGCGcGCGcCGCGCaccgCGUCGGCCa- -3' miRNA: 3'- aaGUGCaUGU-GCGCGa---GCAGCUGGgc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 60733 | 0.66 | 0.89335 |
Target: 5'- -cCGCGUGCuCGUGCUaCGUCcucaacaaGCCCGu -3' miRNA: 3'- aaGUGCAUGuGCGCGA-GCAGc-------UGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 11844 | 0.66 | 0.879624 |
Target: 5'- -cCGCGgGCGCGCGUUCG-CGAgCgCGa -3' miRNA: 3'- aaGUGCaUGUGCGCGAGCaGCUgG-GC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 73790 | 0.66 | 0.879624 |
Target: 5'- --gACGUGCGCGCgGCg-GUCGGCgCGc -3' miRNA: 3'- aagUGCAUGUGCG-CGagCAGCUGgGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 113338 | 0.66 | 0.902416 |
Target: 5'- gUCGCGcgGCACGgGCuguacaacgcgcaguUCGUCG-CCCu -3' miRNA: 3'- aAGUGCa-UGUGCgCG---------------AGCAGCuGGGc -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 95711 | 0.66 | 0.865019 |
Target: 5'- -gCGCGgGCuCGCGCUCG--GGCCCGc -3' miRNA: 3'- aaGUGCaUGuGCGCGAGCagCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 102246 | 0.66 | 0.877489 |
Target: 5'- gUCGCccGCuGCGCGCUCGccuccagcgcccggUCGGCCUGc -3' miRNA: 3'- aAGUGcaUG-UGCGCGAGC--------------AGCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 66447 | 0.66 | 0.872429 |
Target: 5'- -gCGCG-GCGCGCGC-CGcCGGuCCCGc -3' miRNA: 3'- aaGUGCaUGUGCGCGaGCaGCU-GGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 135076 | 0.66 | 0.8866 |
Target: 5'- uUUC-CGgcgGCGgGCUCG-CGGCCCGg -3' miRNA: 3'- -AAGuGCaugUGCgCGAGCaGCUGGGC- -5' |
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23740 | 5' | -56.3 | NC_005261.1 | + | 66736 | 0.66 | 0.899872 |
Target: 5'- cUUCACcauguaguuGUGCACGgGCUgGUCcaccggguguGGCCCGu -3' miRNA: 3'- -AAGUG---------CAUGUGCgCGAgCAG----------CUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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