Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23801 | 5' | -57.5 | NC_005261.1 | + | 125723 | 0.66 | 0.820853 |
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Target: 5'- -gCGGGCucggcuggGGCCGccgCAAGGGGgCcgGGg -3' miRNA: 3'- aaGCCCG--------CCGGCa--GUUCCUCaGuaCC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 127020 | 0.68 | 0.738043 |
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Target: 5'- --gGcGGCGGCCcgagCAGGGAGUCGUc- -3' miRNA: 3'- aagC-CCGCCGGca--GUUCCUCAGUAcc -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 132019 | 0.66 | 0.845692 |
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Target: 5'- -cUGGGCcGCCGUCAcGGA--CGUGGc -3' miRNA: 3'- aaGCCCGcCGGCAGUuCCUcaGUACC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 133185 | 0.68 | 0.728262 |
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Target: 5'- -cCGcGGC-GCCGUCGAGGAGUuCAUc- -3' miRNA: 3'- aaGC-CCGcCGGCAGUUCCUCA-GUAcc -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 135663 | 0.68 | 0.738043 |
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Target: 5'- -gCGGGCGGCCcucgcUCGAGGAGcUCu--- -3' miRNA: 3'- aaGCCCGCCGGc----AGUUCCUC-AGuacc -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 135808 | 0.69 | 0.688406 |
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Target: 5'- -gUGGGCGacgcGCCGuUCGAGGAGUaCAucccUGGg -3' miRNA: 3'- aaGCCCGC----CGGC-AGUUCCUCA-GU----ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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