Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23801 | 5' | -57.5 | NC_005261.1 | + | 33819 | 0.67 | 0.776156 |
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Target: 5'- cUUUGGGCGGCCG---GGGGG-CGcGGg -3' miRNA: 3'- -AAGCCCGCCGGCaguUCCUCaGUaCC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 24181 | 0.66 | 0.837595 |
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Target: 5'- -cCGGGgGGUCGUCGgcgaggcuccAGGAucGUCGgcgGGg -3' miRNA: 3'- aaGCCCgCCGGCAGU----------UCCU--CAGUa--CC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 23727 | 0.71 | 0.556459 |
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Target: 5'- -gCGGGUGGCCGcgcCGAGGcgcGUCcgGGa -3' miRNA: 3'- aaGCCCGCCGGCa--GUUCCu--CAGuaCC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 23641 | 0.7 | 0.627394 |
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Target: 5'- gUCGGGCGGCUGgaAGGGAGagG-GGa -3' miRNA: 3'- aAGCCCGCCGGCagUUCCUCagUaCC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 22696 | 0.73 | 0.415033 |
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Target: 5'- -aCGGGCccggcgacgaGGCCGUCGAGGAGcCGgaGGa -3' miRNA: 3'- aaGCCCG----------CCGGCAGUUCCUCaGUa-CC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 17866 | 0.68 | 0.708463 |
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Target: 5'- -gCGGcCGGCCGgcaggcCAAGGGG-CAUGGu -3' miRNA: 3'- aaGCCcGCCGGCa-----GUUCCUCaGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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