Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23801 | 5' | -57.5 | NC_005261.1 | + | 17866 | 0.68 | 0.708463 |
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Target: 5'- -gCGGcCGGCCGgcaggcCAAGGGG-CAUGGu -3' miRNA: 3'- aaGCCcGCCGGCa-----GUUCCUCaGUACC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 84953 | 0.68 | 0.708463 |
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Target: 5'- cUC-GGCGGCCGcCGAGGcGUCG-GGc -3' miRNA: 3'- aAGcCCGCCGGCaGUUCCuCAGUaCC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 98474 | 0.68 | 0.708463 |
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Target: 5'- -aCGGGCGGCCGUCcuccgcGGGcUCGggcgGGu -3' miRNA: 3'- aaGCCCGCCGGCAGuu----CCUcAGUa---CC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 133185 | 0.68 | 0.728262 |
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Target: 5'- -cCGcGGC-GCCGUCGAGGAGUuCAUc- -3' miRNA: 3'- aaGC-CCGcCGGCAGUUCCUCA-GUAcc -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 90238 | 0.68 | 0.737069 |
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Target: 5'- -gCGGGCGcGCCGUCGgguaucaGGGcgccaaGGUCcUGGa -3' miRNA: 3'- aaGCCCGC-CGGCAGU-------UCC------UCAGuACC- -5' |
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| 23801 | 5' | -57.5 | NC_005261.1 | + | 132019 | 0.66 | 0.845692 |
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Target: 5'- -cUGGGCcGCCGUCAcGGA--CGUGGc -3' miRNA: 3'- aaGCCCGcCGGCAGUuCCUcaGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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