Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23815 | 5' | -54.5 | NC_005261.1 | + | 2996 | 0.66 | 0.961965 |
Target: 5'- gCCugGCGGcCCGGCgccggggCUCccgCGGG-CCu -3' miRNA: 3'- -GGugCGUCuGGCUGa------GAGa--GCUCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 121264 | 0.66 | 0.945956 |
Target: 5'- uCCGcCGCAGAcggggcggccgccCCGGCcucgCUCUCGuccccGUCCc -3' miRNA: 3'- -GGU-GCGUCU-------------GGCUGa---GAGAGCu----CAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 39231 | 0.66 | 0.946392 |
Target: 5'- cUCGCGCGcGCCGGCgaggUCUCcggCGAGgucUCCa -3' miRNA: 3'- -GGUGCGUcUGGCUG----AGAGa--GCUC---AGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 51001 | 0.66 | 0.946392 |
Target: 5'- gCGCGUGGGCgCGGCgggagCUCggCGAGcgCCa -3' miRNA: 3'- gGUGCGUCUG-GCUGa----GAGa-GCUCa-GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 84372 | 0.66 | 0.946392 |
Target: 5'- gCC-CGCGGGCuCGGC-CUCggccgccgccUCGAGcUCCa -3' miRNA: 3'- -GGuGCGUCUG-GCUGaGAG----------AGCUC-AGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 57367 | 0.66 | 0.948113 |
Target: 5'- cCCGCGCGGcCCGACgcccgccagcagCUCGGGgagcgcgCCc -3' miRNA: 3'- -GGUGCGUCuGGCUGaga---------GAGCUCa------GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 51379 | 0.66 | 0.950625 |
Target: 5'- aCGCGCAGGCCGuGCgcgaaCUCcgCGAGcggagCCc -3' miRNA: 3'- gGUGCGUCUGGC-UGa----GAGa-GCUCa----GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 75068 | 0.66 | 0.957298 |
Target: 5'- aCCGCGCGcGCCGGCgggggCUCggcgggcaucgucuUCGGGaCCu -3' miRNA: 3'- -GGUGCGUcUGGCUGa----GAG--------------AGCUCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 80881 | 0.66 | 0.958408 |
Target: 5'- gCCGCGCGGGCuucagCGGCgcCUCccagGGGUCCu -3' miRNA: 3'- -GGUGCGUCUG-----GCUGa-GAGag--CUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 103418 | 0.66 | 0.961619 |
Target: 5'- uCCGCGCGGGCCGcggccacgcgcgcGCgcgC-CUCGcGGUCg -3' miRNA: 3'- -GGUGCGUCUGGC-------------UGa--GaGAGC-UCAGg -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 1601 | 0.66 | 0.961965 |
Target: 5'- gCGCGguGGCUGGCcgcCUCgcccuccgCGaAGUCCg -3' miRNA: 3'- gGUGCguCUGGCUGa--GAGa-------GC-UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 3451 | 0.67 | 0.916057 |
Target: 5'- gCGCGCGGGCCGccGCgccgCgcgUCGAGUaCCg -3' miRNA: 3'- gGUGCGUCUGGC--UGa---Gag-AGCUCA-GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 4321 | 0.67 | 0.916057 |
Target: 5'- gCGCGCGG-CCGcCUCgcggaUCUCGgggcAGUCCc -3' miRNA: 3'- gGUGCGUCuGGCuGAG-----AGAGC----UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 67069 | 0.67 | 0.921705 |
Target: 5'- gCGCGCGGG--GGCUCgCUCGggcGGUCCa -3' miRNA: 3'- gGUGCGUCUggCUGAGaGAGC---UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 23770 | 0.67 | 0.921705 |
Target: 5'- cCCGgGCGGGCCGGCUagggugggCUCgcCGGGgcaggCCg -3' miRNA: 3'- -GGUgCGUCUGGCUGA--------GAGa-GCUCa----GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 23827 | 0.67 | 0.921705 |
Target: 5'- cCCGgGCGGGCCGGCUagggugggCUCgcCGGGgcaggCCg -3' miRNA: 3'- -GGUgCGUCUGGCUGA--------GAGa-GCUCa----GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 80570 | 0.67 | 0.927116 |
Target: 5'- uCCGCG-AGACaGGCUC-CUUGAGgcgCCg -3' miRNA: 3'- -GGUGCgUCUGgCUGAGaGAGCUCa--GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 129368 | 0.67 | 0.927116 |
Target: 5'- gCGCGCGGgucGCgCGGCU---UCGGGUCCa -3' miRNA: 3'- gGUGCGUC---UG-GCUGAgagAGCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 55042 | 0.67 | 0.927116 |
Target: 5'- cCCGCGCAGcugcgcgggGCCGGCgagCUCGGccgCCg -3' miRNA: 3'- -GGUGCGUC---------UGGCUGagaGAGCUca-GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 72937 | 0.67 | 0.939605 |
Target: 5'- gUCGCGCAGcgccggcgccggcACCGGCUCgcgcccgccgccgCggGGGUCCg -3' miRNA: 3'- -GGUGCGUC-------------UGGCUGAGa------------GagCUCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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