Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23815 | 5' | -54.5 | NC_005261.1 | + | 73825 | 0.78 | 0.408133 |
Target: 5'- cCCGCGCcuAGcCCGGCUCUUgggCGAGUCg -3' miRNA: 3'- -GGUGCG--UCuGGCUGAGAGa--GCUCAGg -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 28481 | 0.76 | 0.480411 |
Target: 5'- gCCACGCucGACCuguacGAC-CUCaUCGAGUCCg -3' miRNA: 3'- -GGUGCGu-CUGG-----CUGaGAG-AGCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 68998 | 0.74 | 0.588602 |
Target: 5'- gCCGCGCGGGCgccgCGGCUUcgUUggCGAGUCCg -3' miRNA: 3'- -GGUGCGUCUG----GCUGAG--AGa-GCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 117113 | 0.73 | 0.639502 |
Target: 5'- aCGCGCGGcgcugaccuuGCCGugUCgcUCUCGAGgCCg -3' miRNA: 3'- gGUGCGUC----------UGGCugAG--AGAGCUCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 100525 | 0.73 | 0.669018 |
Target: 5'- gCCGCGCGGcGCCaGCgcgucgaUCUC-CGAGUCCg -3' miRNA: 3'- -GGUGCGUC-UGGcUG-------AGAGaGCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 67832 | 0.73 | 0.670032 |
Target: 5'- gCGCGCAGgaggGCCGcACUCUC-CGGG-CCg -3' miRNA: 3'- gGUGCGUC----UGGC-UGAGAGaGCUCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 32550 | 0.72 | 0.706284 |
Target: 5'- gCgGCGCGGGCCGGCgcgcguggaagugCUCUCcucGUCCg -3' miRNA: 3'- -GgUGCGUCUGGCUGa------------GAGAGcu-CAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 16565 | 0.71 | 0.749451 |
Target: 5'- -gGCGCAgGACCuuGCUCUgCUCGuAGUCCu -3' miRNA: 3'- ggUGCGU-CUGGc-UGAGA-GAGC-UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 1484 | 0.71 | 0.772211 |
Target: 5'- gCCGCGCAGaaggccaccgccgcgGCCGGCagCUCgUCGGGcgCCa -3' miRNA: 3'- -GGUGCGUC---------------UGGCUGa-GAG-AGCUCa-GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 66542 | 0.71 | 0.772211 |
Target: 5'- aCGCGCAGcagcuggucggccguGCCGcGCUCgaaCGAGUCCa -3' miRNA: 3'- gGUGCGUC---------------UGGC-UGAGagaGCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 118107 | 0.71 | 0.786995 |
Target: 5'- gCCGCGCAGacgcugcgccGCCGGCUCgcgccgCUCG-G-CCg -3' miRNA: 3'- -GGUGCGUC----------UGGCUGAGa-----GAGCuCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 104844 | 0.7 | 0.822328 |
Target: 5'- gCCACGCGGACCG-Cg----CGGGUCa -3' miRNA: 3'- -GGUGCGUCUGGCuGagagaGCUCAGg -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 131232 | 0.69 | 0.838997 |
Target: 5'- gCCGCGCgcaucgAGGCUGAC-CUCgcCGAGgUCCg -3' miRNA: 3'- -GGUGCG------UCUGGCUGaGAGa-GCUC-AGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 32065 | 0.69 | 0.847056 |
Target: 5'- gCCGCGCAGAgCgCGGCgcuggggCUCGGGgagCCg -3' miRNA: 3'- -GGUGCGUCU-G-GCUGaga----GAGCUCa--GG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 95306 | 0.69 | 0.862584 |
Target: 5'- -gGCGCAGACgGACUgCgaccccuacgUC-CGGGUCCa -3' miRNA: 3'- ggUGCGUCUGgCUGA-G----------AGaGCUCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 39294 | 0.69 | 0.868567 |
Target: 5'- gCCACGguGGCgGACUacacgcgguucCUCGcGUCCa -3' miRNA: 3'- -GGUGCguCUGgCUGAga---------GAGCuCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 50633 | 0.69 | 0.869305 |
Target: 5'- uCCACGCGGgcggcgggguggcGCCGGCgaaCgcgCUCGcccaAGUCCg -3' miRNA: 3'- -GGUGCGUC-------------UGGCUGa--Ga--GAGC----UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 95908 | 0.68 | 0.877287 |
Target: 5'- cCCGCGCGG-CgCG-CUCaCUCGcGGUCCg -3' miRNA: 3'- -GGUGCGUCuG-GCuGAGaGAGC-UCAGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 74946 | 0.68 | 0.884315 |
Target: 5'- gCgGCGCGGGCgCG-CUCggcgcgCUCGAGgCCg -3' miRNA: 3'- -GgUGCGUCUG-GCuGAGa-----GAGCUCaGG- -5' |
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23815 | 5' | -54.5 | NC_005261.1 | + | 95709 | 0.68 | 0.891122 |
Target: 5'- gCGCGCGGGCUcGCg--CUCGGGcCCg -3' miRNA: 3'- gGUGCGUCUGGcUGagaGAGCUCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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