Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23837 | 5' | -55.9 | NC_005261.1 | + | 34909 | 0.66 | 0.890003 |
Target: 5'- gACgCGGAgGUCGGgGcgcggccacGCGGGGGGCg -3' miRNA: 3'- aUGaGUCUgCAGUCgC---------UGUUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 43259 | 0.66 | 0.887928 |
Target: 5'- cGCUCguugagugcguucgGGGCGUC-GCGGCcgccuuucccgcGGGGGGCg -3' miRNA: 3'- aUGAG--------------UCUGCAGuCGCUG------------UUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 115635 | 0.66 | 0.883004 |
Target: 5'- aGCgCAG-CGUCGGgGGgaaGAGGGGCg -3' miRNA: 3'- aUGaGUCuGCAGUCgCUg--UUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 4524 | 0.66 | 0.868319 |
Target: 5'- cGCgCGGGCG-CcGCGGCGAGGGcGCc -3' miRNA: 3'- aUGaGUCUGCaGuCGCUGUUCCC-CGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 5746 | 0.66 | 0.860643 |
Target: 5'- cGCgcgCGGAaggugaggGUUAGUGugGGGGGGCUu -3' miRNA: 3'- aUGa--GUCUg-------CAGUCGCugUUCCCCGA- -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 38254 | 0.66 | 0.852753 |
Target: 5'- gGCUCGcACGcCAGCcugGGCuGGGGGCg -3' miRNA: 3'- aUGAGUcUGCaGUCG---CUGuUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 98288 | 0.66 | 0.852753 |
Target: 5'- cGCcCGGcGCGUCcGCGGCcucGGGGGCg -3' miRNA: 3'- aUGaGUC-UGCAGuCGCUGu--UCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 58280 | 0.67 | 0.844655 |
Target: 5'- cGCUCAcGGgGcCGGCGcgcCGGGGGGCg -3' miRNA: 3'- aUGAGU-CUgCaGUCGCu--GUUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 90067 | 0.67 | 0.836358 |
Target: 5'- gGCgccccgCGGGCGccgagggCAGCGACG-GGGGCc -3' miRNA: 3'- aUGa-----GUCUGCa------GUCGCUGUuCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 63967 | 0.67 | 0.836358 |
Target: 5'- aGCUgGuGCGUCAGCGcCAGcGGGCUc -3' miRNA: 3'- aUGAgUcUGCAGUCGCuGUUcCCCGA- -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 59450 | 0.67 | 0.827868 |
Target: 5'- gGCagCAGcGCGgccgCGGCGuCGGGGGGCa -3' miRNA: 3'- aUGa-GUC-UGCa---GUCGCuGUUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 98916 | 0.67 | 0.827868 |
Target: 5'- gGgUCGGcgGgGUCGGCuGCGGGGGGCa -3' miRNA: 3'- aUgAGUC--UgCAGUCGcUGUUCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 110222 | 0.68 | 0.792143 |
Target: 5'- -cCUCGGcgacgccuccaGCGUgGGCGGCGcggcgcccgGGGGGCUg -3' miRNA: 3'- auGAGUC-----------UGCAgUCGCUGU---------UCCCCGA- -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 101418 | 0.68 | 0.792143 |
Target: 5'- uUGCgUCAcGACGUCGGcCGcCGAGGcGGCg -3' miRNA: 3'- -AUG-AGU-CUGCAGUC-GCuGUUCC-CCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 44313 | 0.68 | 0.792143 |
Target: 5'- cUGCccgCGGGCG-CcGCaGACGGGGGGCUg -3' miRNA: 3'- -AUGa--GUCUGCaGuCG-CUGUUCCCCGA- -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 70578 | 0.68 | 0.791217 |
Target: 5'- aGCUCGGGCGcgagcgCAGCGGCGugcgcccgcccgaAGaGGGCc -3' miRNA: 3'- aUGAGUCUGCa-----GUCGCUGU-------------UC-CCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 129647 | 0.68 | 0.782815 |
Target: 5'- aACgguGGCGgCGGCGGCGgcAGGGGCg -3' miRNA: 3'- aUGaguCUGCaGUCGCUGU--UCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 80875 | 0.68 | 0.782815 |
Target: 5'- cGCUCAGcCGcgcgggcuUCAGCGGCGccucccAGGGGUc -3' miRNA: 3'- aUGAGUCuGC--------AGUCGCUGU------UCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 1395 | 0.68 | 0.773347 |
Target: 5'- cGC-CGGGCGccgcggccgCGGCGGCGgcGGGGGCg -3' miRNA: 3'- aUGaGUCUGCa--------GUCGCUGU--UCCCCGa -5' |
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23837 | 5' | -55.9 | NC_005261.1 | + | 130475 | 0.68 | 0.763748 |
Target: 5'- gGCUCGcGGCGugccUCGGCGGCGAGGGc-- -3' miRNA: 3'- aUGAGU-CUGC----AGUCGCUGUUCCCcga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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