Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23935 | 5' | -54.4 | NC_005262.1 | + | 50422 | 0.66 | 0.795522 |
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Target: 5'- cGgAGCGCGAGggccgcgugcacguCGUCGUGCcgcgCGaGGAg -3' miRNA: 3'- -CgUCGCGCUC--------------GCAGCAUGaaa-GC-CCU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 58561 | 0.66 | 0.807865 |
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Target: 5'- uCAGCGCG-GCGUCG-ACgccaCGGcGAu -3' miRNA: 3'- cGUCGCGCuCGCAGCaUGaaa-GCC-CU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 17330 | 0.66 | 0.817153 |
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Target: 5'- aGgAGCGCGAGCG-CGU-Cg--CGGcGAu -3' miRNA: 3'- -CgUCGCGCUCGCaGCAuGaaaGCC-CU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 46318 | 0.66 | 0.826251 |
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Target: 5'- cGCGGCGCGcGCuUCGUcCUggaacUCGGcGAa -3' miRNA: 3'- -CGUCGCGCuCGcAGCAuGAa----AGCC-CU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 18104 | 0.66 | 0.826251 |
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Target: 5'- aGCGGC-CG-GCGUCG-ACaaUUGGGAg -3' miRNA: 3'- -CGUCGcGCuCGCAGCaUGaaAGCCCU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 12807 | 0.66 | 0.829835 |
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Target: 5'- gGCGGCGCGcagccGGCG-CGUGaagaaggagcgcggcCUcgCGGGAa -3' miRNA: 3'- -CGUCGCGC-----UCGCaGCAU---------------GAaaGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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