Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24331 | 5' | -58.5 | NC_005264.1 | + | 75106 | 0.71 | 0.55839 |
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Target: 5'- gCGCCgGcGCCGaGCUGGGGCGCagucaaaugGCCGc -3' miRNA: 3'- -GUGGgU-CGGCaUGACCUCGCGa--------UGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 101586 | 0.72 | 0.490982 |
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Target: 5'- aCGCCgAggcgcGCCGUgaGCUGGAGCGC-GCCa -3' miRNA: 3'- -GUGGgU-----CGGCA--UGACCUCGCGaUGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 29118 | 0.72 | 0.463247 |
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Target: 5'- aACCCcccgaGGCUGUACcugaGGAGCGCgugGCCGc -3' miRNA: 3'- gUGGG-----UCGGCAUGa---CCUCGCGa--UGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 42937 | 0.73 | 0.427593 |
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Target: 5'- aCACCCAGUCGUACguccGGGCGCggcaacGCCu -3' miRNA: 3'- -GUGGGUCGGCAUGac--CUCGCGa-----UGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 161964 | 0.73 | 0.427593 |
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Target: 5'- aCACCCAGUCGUACguccGGGCGCggcaacGCCu -3' miRNA: 3'- -GUGGGUCGGCAUGac--CUCGCGa-----UGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 55935 | 0.76 | 0.302747 |
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Target: 5'- gACCCGGCUucugGaACUGGGuGCGCUACCGc -3' miRNA: 3'- gUGGGUCGG----CaUGACCU-CGCGAUGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 88585 | 0.76 | 0.302747 |
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Target: 5'- cCGCCUcuaccGCCGUGuCUGGAGCGCgGCCa -3' miRNA: 3'- -GUGGGu----CGGCAU-GACCUCGCGaUGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 162044 | 1.07 | 0.002382 |
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Target: 5'- gCACCCAGCCGUACUGGAGCGCUACCGc -3' miRNA: 3'- -GUGGGUCGGCAUGACCUCGCGAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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