Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24331 | 5' | -58.5 | NC_005264.1 | + | 98669 | 0.68 | 0.717623 |
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Target: 5'- gACCCAGU---GCUGGcGGCGCUugCa -3' miRNA: 3'- gUGGGUCGgcaUGACC-UCGCGAugGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 98980 | 0.67 | 0.746485 |
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Target: 5'- ---aCGGCCGUugUGGAGCG--GCCu -3' miRNA: 3'- guggGUCGGCAugACCUCGCgaUGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 99771 | 0.67 | 0.783578 |
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Target: 5'- gGCCCucGCCGaGCUGGAGaccgagaucaaGCgGCCGa -3' miRNA: 3'- gUGGGu-CGGCaUGACCUCg----------CGaUGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 104939 | 0.67 | 0.783578 |
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Target: 5'- -cCCCAGCCGUGCca-AGCGCcgaGCCu -3' miRNA: 3'- guGGGUCGGCAUGaccUCGCGa--UGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 24949 | 0.66 | 0.810057 |
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Target: 5'- gCACaUguGCCGUACcaGGGCGCUgcGCCGa -3' miRNA: 3'- -GUG-GguCGGCAUGacCUCGCGA--UGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 157569 | 0.66 | 0.810057 |
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Target: 5'- aCGCCacgaGGCCGgGCUuGGGCGCUuccCCGu -3' miRNA: 3'- -GUGGg---UCGGCaUGAcCUCGCGAu--GGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 138774 | 0.66 | 0.818583 |
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Target: 5'- gCGCCUGGCCGUGCUGuuG-GCUGuguCCGc -3' miRNA: 3'- -GUGGGUCGGCAUGACcuCgCGAU---GGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 162044 | 1.07 | 0.002382 |
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Target: 5'- gCACCCAGCCGUACUGGAGCGCUACCGc -3' miRNA: 3'- -GUGGGUCGGCAUGACCUCGCGAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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