Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24331 | 5' | -58.5 | NC_005264.1 | + | 55135 | 0.67 | 0.792546 |
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Target: 5'- aCACCCcGCCGUGCgcu-GUGCUGgCGc -3' miRNA: 3'- -GUGGGuCGGCAUGaccuCGCGAUgGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 44631 | 0.67 | 0.792546 |
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Target: 5'- uCACCgCGGCCGag--GGAGUGCUgauguagaagcuGCCGu -3' miRNA: 3'- -GUGG-GUCGGCaugaCCUCGCGA------------UGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 24949 | 0.66 | 0.810057 |
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Target: 5'- gCACaUguGCCGUACcaGGGCGCUgcGCCGa -3' miRNA: 3'- -GUG-GguCGGCAUGacCUCGCGA--UGGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 157569 | 0.66 | 0.810057 |
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Target: 5'- aCGCCacgaGGCCGgGCUuGGGCGCUuccCCGu -3' miRNA: 3'- -GUGGg---UCGGCaUGAcCUCGCGAu--GGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 66067 | 0.66 | 0.810057 |
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Target: 5'- aGCCCGGgaauaaguuucUCGUAgucuCUGGGGgGCUGCCc -3' miRNA: 3'- gUGGGUC-----------GGCAU----GACCUCgCGAUGGc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 138774 | 0.66 | 0.818583 |
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Target: 5'- gCGCCUGGCCGUGCUGuuG-GCUGuguCCGc -3' miRNA: 3'- -GUGGGUCGGCAUGACcuCgCGAU---GGC- -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 98472 | 0.66 | 0.83514 |
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Target: 5'- aACCCGGCCGgcacGCgcgagGGcaucuccucAGCGCUACa- -3' miRNA: 3'- gUGGGUCGGCa---UGa----CC---------UCGCGAUGgc -5' |
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| 24331 | 5' | -58.5 | NC_005264.1 | + | 76693 | 0.66 | 0.83514 |
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Target: 5'- uGCCCAGCUugGCgcGGAGCGCgGCg- -3' miRNA: 3'- gUGGGUCGGcaUGa-CCUCGCGaUGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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