Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24347 | 3' | -51.6 | NC_005264.1 | + | 29097 | 0.66 | 0.982128 |
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Target: 5'- uGCGUAuaggcucccAGCGCcgcAAGGGCUGUCgUGcCa -3' miRNA: 3'- -CGCGU---------UCGCGa--UUCUCGAUAGgACuG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 18147 | 0.75 | 0.654744 |
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Target: 5'- gGCGCGAGCgGCUAAGAGacaggcuUGUCggCUGACg -3' miRNA: 3'- -CGCGUUCG-CGAUUCUCg------AUAG--GACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 17045 | 0.71 | 0.881106 |
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Target: 5'- aGCGCGGcCGCUAAGAGCaugCC-GACc -3' miRNA: 3'- -CGCGUUcGCGAUUCUCGauaGGaCUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 14392 | 0.74 | 0.71778 |
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Target: 5'- uCGCGGGCGCc---GGCUGUUCUGACc -3' miRNA: 3'- cGCGUUCGCGauucUCGAUAGGACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 10560 | 0.73 | 0.768221 |
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Target: 5'- uGCGCAGGCGCgcaacauaAGCaaaAUCCUGGCc -3' miRNA: 3'- -CGCGUUCGCGauuc----UCGa--UAGGACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 9937 | 0.7 | 0.914649 |
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Target: 5'- uUGCGGcGCGCUGAGAGCUAUaCU-ACa -3' miRNA: 3'- cGCGUU-CGCGAUUCUCGAUAgGAcUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 3819 | 0.67 | 0.969269 |
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Target: 5'- gGUGCucccuGCGCgcgGAGAcCUGcUCCUGGCa -3' miRNA: 3'- -CGCGuu---CGCGa--UUCUcGAU-AGGACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 2510 | 0.67 | 0.972255 |
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Target: 5'- cGCGCGacuucAGCGCgGGGGGCgacuUCCcgaGACg -3' miRNA: 3'- -CGCGU-----UCGCGaUUCUCGau--AGGa--CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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