Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24348 | 3' | -56.5 | NC_005264.1 | + | 1615 | 0.66 | 0.887893 |
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Target: 5'- aUACGGGacg-CC-GGugGGCGcGGCUCc -3' miRNA: 3'- -AUGCCCguuaGGaCCugCUGC-UCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 119117 | 0.66 | 0.880951 |
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Target: 5'- gGCGGGCucugCUgggGGAgGGCGAGgaCg -3' miRNA: 3'- aUGCCCGuua-GGa--CCUgCUGCUCgaG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 66497 | 0.66 | 0.880951 |
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Target: 5'- gGCGGGCugcgc-GGcgcgcuCGACGAGCUCg -3' miRNA: 3'- aUGCCCGuuaggaCCu-----GCUGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 80756 | 0.66 | 0.865659 |
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Target: 5'- --gGGGaCAAUCCUcGGCGGCGAagaucuuguccgcGCUCu -3' miRNA: 3'- augCCC-GUUAGGAcCUGCUGCU-------------CGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 19025 | 0.67 | 0.858817 |
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Target: 5'- aGCGGGCGuga-UGGACGGCaGGCg- -3' miRNA: 3'- aUGCCCGUuaggACCUGCUGcUCGag -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 3330 | 0.67 | 0.851021 |
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Target: 5'- gACGGGCAAUaCUGGccuUGGCGAgggucuuucGCUCc -3' miRNA: 3'- aUGCCCGUUAgGACCu--GCUGCU---------CGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 13243 | 0.67 | 0.850231 |
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Target: 5'- aGCaGGUggUCCUcuaccgacucggaGGACGACGAggGUUCg -3' miRNA: 3'- aUGcCCGuuAGGA-------------CCUGCUGCU--CGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 145662 | 1.07 | 0.003149 |
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Target: 5'- gUACGGGCAAUCCUGGACGACGAGCUCu -3' miRNA: 3'- -AUGCCCGUUAGGACCUGCUGCUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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