Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24348 | 3' | -56.5 | NC_005264.1 | + | 61824 | 0.66 | 0.880951 |
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Target: 5'- -cCGGGCAGUgCCUGGcucgccugcgccGCGuACGGGUUg -3' miRNA: 3'- auGCCCGUUA-GGACC------------UGC-UGCUCGAg -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 66497 | 0.66 | 0.880951 |
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Target: 5'- gGCGGGCugcgc-GGcgcgcuCGACGAGCUCg -3' miRNA: 3'- aUGCCCGuuaggaCCu-----GCUGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 120642 | 0.66 | 0.887893 |
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Target: 5'- aUACGGGacg-CC-GGugGGCGcGGCUCc -3' miRNA: 3'- -AUGCCCguuaGGaCCugCUGC-UCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 1615 | 0.66 | 0.887893 |
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Target: 5'- aUACGGGacg-CC-GGugGGCGcGGCUCc -3' miRNA: 3'- -AUGCCCguuaGGaCCugCUGC-UCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 145936 | 0.66 | 0.901095 |
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Target: 5'- cGCGGGCAugggCCUcaccGACGACG-GCg- -3' miRNA: 3'- aUGCCCGUua--GGAc---CUGCUGCuCGag -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 108977 | 0.66 | 0.901095 |
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Target: 5'- cACGGGguAcgacgcgucucUCCuagccuUGaACGACGAGCUCa -3' miRNA: 3'- aUGCCCguU-----------AGG------ACcUGCUGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 44992 | 0.66 | 0.901095 |
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Target: 5'- --aGGGCGAgCCUGGcgagaacgGCGACGAGa-- -3' miRNA: 3'- augCCCGUUaGGACC--------UGCUGCUCgag -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 16566 | 0.66 | 0.901095 |
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Target: 5'- -gUGGGCGGcgCCgGGGCGGCGGcGCa- -3' miRNA: 3'- auGCCCGUUa-GGaCCUGCUGCU-CGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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