Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24348 | 3' | -56.5 | NC_005264.1 | + | 15223 | 0.7 | 0.674328 |
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Target: 5'- gACGcGGCGAUCCUGcGcucuaaguucaACG-CGGGCUCg -3' miRNA: 3'- aUGC-CCGUUAGGAC-C-----------UGCuGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 157361 | 0.7 | 0.664141 |
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Target: 5'- gGCGGGCccuggccguGAUgCUGGGCGGCgGGGCg- -3' miRNA: 3'- aUGCCCG---------UUAgGACCUGCUG-CUCGag -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 38334 | 0.7 | 0.664141 |
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Target: 5'- gGCGGGCccuggccguGAUgCUGGGCGGCgGGGCg- -3' miRNA: 3'- aUGCCCG---------UUAgGACCUGCUG-CUCGag -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 100535 | 0.71 | 0.633459 |
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Target: 5'- gGCGGGUcuAGUCC-GGACGAC--GCUCg -3' miRNA: 3'- aUGCCCG--UUAGGaCCUGCUGcuCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 128774 | 0.73 | 0.522362 |
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Target: 5'- cUGCGGGCuuccuucuucucGGUUCUGGuuugugGCGACGAGUUCc -3' miRNA: 3'- -AUGCCCG------------UUAGGACC------UGCUGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 9748 | 0.73 | 0.522362 |
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Target: 5'- cUGCGGGCuuccuucuucucGGUUCUGGuuugugGCGACGAGUUCc -3' miRNA: 3'- -AUGCCCG------------UUAGGACC------UGCUGCUCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 123479 | 0.75 | 0.405654 |
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Target: 5'- gUACGGGCAugcgguggccgcgcgGUUgCUGGGCGAUGuGCUCg -3' miRNA: 3'- -AUGCCCGU---------------UAG-GACCUGCUGCuCGAG- -5' |
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| 24348 | 3' | -56.5 | NC_005264.1 | + | 145662 | 1.07 | 0.003149 |
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Target: 5'- gUACGGGCAAUCCUGGACGACGAGCUCu -3' miRNA: 3'- -AUGCCCGUUAGGACCUGCUGCUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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