Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24359 | 5' | -56.9 | NC_005264.1 | + | 93481 | 0.72 | 0.537803 |
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Target: 5'- -cGCUGUCUgaGGCGCuGCUGGCggcCGGc -3' miRNA: 3'- aaUGAUAGGg-CCGCG-CGACCGaa-GCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 122094 | 0.72 | 0.537803 |
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Target: 5'- -cGCUGUCUCGcCGCGaCUGGCggCGGc -3' miRNA: 3'- aaUGAUAGGGCcGCGC-GACCGaaGCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 3067 | 0.72 | 0.537803 |
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Target: 5'- -cGCUGUCUCGcCGCGaCUGGCggCGGc -3' miRNA: 3'- aaUGAUAGGGCcGCGC-GACCGaaGCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 34446 | 0.76 | 0.335224 |
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Target: 5'- gUUAgUGUCCCGGgGCGUUGGagguggUCGGg -3' miRNA: 3'- -AAUgAUAGGGCCgCGCGACCga----AGCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 153473 | 0.76 | 0.335224 |
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Target: 5'- gUUAgUGUCCCGGgGCGUUGGagguggUCGGg -3' miRNA: 3'- -AAUgAUAGGGCCgCGCGACCga----AGCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 53274 | 0.82 | 0.151074 |
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Target: 5'- -cGCUAgaUCCUGGcCGCGCgGGCUUCGGg -3' miRNA: 3'- aaUGAU--AGGGCC-GCGCGaCCGAAGCC- -5' |
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| 24359 | 5' | -56.9 | NC_005264.1 | + | 138621 | 1.07 | 0.0033 |
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Target: 5'- cUUACUAUCCCGGCGCGCUGGCUUCGGc -3' miRNA: 3'- -AAUGAUAGGGCCGCGCGACCGAAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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