Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24612 | 5' | -54.5 | NC_005264.1 | + | 17976 | 0.69 | 0.859143 |
Target: 5'- cCGAUGGCGagcgcguUCaaaGCCGgcacCGGCUCUGACg -3' miRNA: 3'- -GCUACUGC-------AGa--CGGC----GCCGAGAUUGa -5' |
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24612 | 5' | -54.5 | NC_005264.1 | + | 17340 | 0.69 | 0.844122 |
Target: 5'- ---gGACGUCUGCagugccuucCGCGGCagCUGGCg -3' miRNA: 3'- gcuaCUGCAGACG---------GCGCCGa-GAUUGa -5' |
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24612 | 5' | -54.5 | NC_005264.1 | + | 15593 | 0.67 | 0.920134 |
Target: 5'- uGcgGGCG-CaGCUGCGGCUCcGGCa -3' miRNA: 3'- gCuaCUGCaGaCGGCGCCGAGaUUGa -5' |
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24612 | 5' | -54.5 | NC_005264.1 | + | 5504 | 0.72 | 0.729408 |
Target: 5'- gCGAUGAUGUUUguuggccugaugacaGCCGCGGCgUCUcGCg -3' miRNA: 3'- -GCUACUGCAGA---------------CGGCGCCG-AGAuUGa -5' |
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24612 | 5' | -54.5 | NC_005264.1 | + | 2646 | 0.66 | 0.953733 |
Target: 5'- aGGUuGCGcUCUGCaGCGGCagcUCUGACg -3' miRNA: 3'- gCUAcUGC-AGACGgCGCCG---AGAUUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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