Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24810 | 3' | -56.6 | NC_005284.1 | + | 43820 | 0.66 | 0.651429 |
Target: 5'- uAAGCG--GGa---CCGCCGUGCGCc -3' miRNA: 3'- cUUCGCuaCCguuaGGCGGCACGCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 27589 | 0.66 | 0.640472 |
Target: 5'- aAAGCGAaggccgagGGCAAagcgCCGCCGauuaUGCGUa -3' miRNA: 3'- cUUCGCUa-------CCGUUa---GGCGGC----ACGCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 21328 | 0.66 | 0.629506 |
Target: 5'- cGGGCGAcUGGCGGggCGCgUGUcGCGCGa -3' miRNA: 3'- cUUCGCU-ACCGUUagGCG-GCA-CGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 6568 | 0.66 | 0.629506 |
Target: 5'- --uGCGAaGGUGAUCCGUgaCGagGCGCGu -3' miRNA: 3'- cuuCGCUaCCGUUAGGCG--GCa-CGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 53651 | 0.66 | 0.618544 |
Target: 5'- uGGAGCacGAUGGCGAcgCGCCGU-CGCc -3' miRNA: 3'- -CUUCG--CUACCGUUagGCGGCAcGCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 30358 | 0.66 | 0.596662 |
Target: 5'- cGAGGCGAUcgagcgcgagGGCGAUgCGCucggcccgCGUGUGCu -3' miRNA: 3'- -CUUCGCUA----------CCGUUAgGCG--------GCACGCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 32971 | 0.66 | 0.595571 |
Target: 5'- gGAAGCGccgGGCAcgCCgucgccaccggcuGCCGUGCaGUGu -3' miRNA: 3'- -CUUCGCua-CCGUuaGG-------------CGGCACG-CGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 26505 | 0.67 | 0.590118 |
Target: 5'- -uGGCGAUccagucugaugcaucGGCAGUUCGCCGcGcCGCu -3' miRNA: 3'- cuUCGCUA---------------CCGUUAGGCGGCaC-GCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 1376 | 0.67 | 0.585762 |
Target: 5'- aGggGaCGAUuGGacCGAUCCGCgCGUGCugGCGa -3' miRNA: 3'- -CuuC-GCUA-CC--GUUAGGCG-GCACG--CGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 49322 | 0.67 | 0.5749 |
Target: 5'- -cGGCGAUGGUuggcaCgGCacgCGUGCGCGa -3' miRNA: 3'- cuUCGCUACCGuua--GgCG---GCACGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 38575 | 0.67 | 0.553325 |
Target: 5'- cGAGCGAgacGGCcguUCgGCCGaGCGCa -3' miRNA: 3'- cUUCGCUa--CCGuu-AGgCGGCaCGCGc -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 51413 | 0.67 | 0.553325 |
Target: 5'- gGAAGUGcgGGCAcagugugCUGCCGUggugaGUGCGg -3' miRNA: 3'- -CUUCGCuaCCGUua-----GGCGGCA-----CGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 8513 | 0.67 | 0.553325 |
Target: 5'- --cGCuGAUGGCu-UCCGCCGacaucggacGCGCGu -3' miRNA: 3'- cuuCG-CUACCGuuAGGCGGCa--------CGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 38972 | 0.67 | 0.542627 |
Target: 5'- --cGCGAUGcUGAUCCGCgGgcUGCGCGc -3' miRNA: 3'- cuuCGCUACcGUUAGGCGgC--ACGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 45642 | 0.67 | 0.542627 |
Target: 5'- cGggGCaGGcUGGCGGcUCGUCGgUGCGCGa -3' miRNA: 3'- -CuuCG-CU-ACCGUUaGGCGGC-ACGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 6817 | 0.68 | 0.531998 |
Target: 5'- cGAGGcCGAgggGGCGAUUCGCaccgaGUugGCGCGc -3' miRNA: 3'- -CUUC-GCUa--CCGUUAGGCGg----CA--CGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 12322 | 0.68 | 0.490303 |
Target: 5'- uGAGCGAUugaacgcagggaGGCGAUCCGgCUucGCGCGa -3' miRNA: 3'- cUUCGCUA------------CCGUUAGGC-GGcaCGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 15477 | 0.68 | 0.480114 |
Target: 5'- --cGCGAcUGGUAucUCGCCGaGCGCGg -3' miRNA: 3'- cuuCGCU-ACCGUuaGGCGGCaCGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 10640 | 0.68 | 0.479101 |
Target: 5'- cGAGCGgcGGCGAagCGCUgcgcgaggaacugGUGCGCGc -3' miRNA: 3'- cUUCGCuaCCGUUagGCGG-------------CACGCGC- -5' |
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24810 | 3' | -56.6 | NC_005284.1 | + | 9751 | 0.69 | 0.47003 |
Target: 5'- cGAAGCGcgGGCGGUCgaGgCGacgGCGCa -3' miRNA: 3'- -CUUCGCuaCCGUUAGg-CgGCa--CGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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