Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24824 | 3' | -53.8 | NC_005284.1 | + | 15268 | 0.66 | 0.826116 |
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Target: 5'- ------aUCCCGGcgcaucGGCacaGCCGACCg -3' miRNA: 3'- ggucuuaAGGGCCau----UCGg--CGGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 14821 | 0.71 | 0.542627 |
|
Target: 5'- uCCAGuggaaUCgaaaCGG-AAGCUGCCGACCg -3' miRNA: 3'- -GGUCuua--AGg---GCCaUUCGGCGGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 7126 | 0.66 | 0.816949 |
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Target: 5'- uUCAGGAUcgguUCUCGGUcgGAGgUGUCGACg -3' miRNA: 3'- -GGUCUUA----AGGGCCA--UUCgGCGGCUGg -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 5439 | 0.67 | 0.787352 |
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Target: 5'- uCCGGAAUcguuugugaCCGGUGcauuagucgagcaAGCCGgCGGCUg -3' miRNA: 3'- -GGUCUUAag-------GGCCAU-------------UCGGCgGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 5346 | 0.67 | 0.778457 |
|
Target: 5'- -aAGAGUggCCGGUcgAGGUCGCCGGa- -3' miRNA: 3'- ggUCUUAagGGCCA--UUCGGCGGCUgg -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 150 | 0.68 | 0.684155 |
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Target: 5'- gCCAGAcuucggCuuGGUcacgaacaucGAGCCGCCgGACUg -3' miRNA: 3'- -GGUCUuaa---GggCCA----------UUCGGCGG-CUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 112 | 0.68 | 0.716435 |
|
Target: 5'- gCgGGAAaUCCCGGUAAacGCgCGCUGAa- -3' miRNA: 3'- -GgUCUUaAGGGCCAUU--CG-GCGGCUgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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