Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25029 | 5' | -52.9 | NC_005336.1 | + | 137226 | 0.66 | 0.969125 |
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Target: 5'- aGAGGACGAGaGaacggauaGCGAGUCGAC-AGUc -3' miRNA: 3'- -CUCUUGCUCaC--------UGCUCAGCUGcUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 137226 | 0.66 | 0.969125 |
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Target: 5'- aGAGGACGAGaGaacggauaGCGAGUCGAC-AGUc -3' miRNA: 3'- -CUCUUGCUCaC--------UGCUCAGCUGcUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 7487 | 0.66 | 0.97205 |
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Target: 5'- -cGGGCGcGcGGCG-GUCGcACGAGCGg -3' miRNA: 3'- cuCUUGCuCaCUGCuCAGC-UGCUCGC- -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 22020 | 0.66 | 0.97205 |
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Target: 5'- aAGAGCGGcUGG-GGGUCGuCGAGCa -3' miRNA: 3'- cUCUUGCUcACUgCUCAGCuGCUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 101654 | 0.66 | 0.974771 |
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Target: 5'- cGAGGA-GGGccGCGAGUgCGugGAGCa -3' miRNA: 3'- -CUCUUgCUCacUGCUCA-GCugCUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 77970 | 0.66 | 0.977049 |
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Target: 5'- uGGGGCGAGgggGugcgcuuCGAGUCGccgcccuGCGAGCa -3' miRNA: 3'- cUCUUGCUCa--Cu------GCUCAGC-------UGCUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 10598 | 0.66 | 0.977293 |
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Target: 5'- -uGAGCGAGgggagGACGAccgCGGCGGcGCGc -3' miRNA: 3'- cuCUUGCUCa----CUGCUca-GCUGCU-CGC- -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 55143 | 0.66 | 0.978248 |
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Target: 5'- aGAGAGCGccacgucgcugacUGAgGAGUCGGCG-GCGc -3' miRNA: 3'- -CUCUUGCuc-----------ACUgCUCAGCUGCuCGC- -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 132581 | 0.66 | 0.979624 |
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Target: 5'- -cGAccGCGAGUGGCcggacuGUCGcGCGAGCu -3' miRNA: 3'- cuCU--UGCUCACUGcu----CAGC-UGCUCGc -5' |
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| 25029 | 5' | -52.9 | NC_005336.1 | + | 123731 | 0.66 | 0.979624 |
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Target: 5'- -cGGGCGAGUG-CGGGcaUCGugcCGAGCu -3' miRNA: 3'- cuCUUGCUCACuGCUC--AGCu--GCUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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