Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25981 | 3' | -55.1 | NC_005340.1 | + | 4966 | 0.66 | 0.53113 |
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Target: 5'- uCAUCCGCguuucugaaaacGAUUACAuUCuGCCGGuCGCa -3' miRNA: 3'- -GUAGGCGa-----------CUGGUGUuAG-UGGCC-GCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 2843 | 0.72 | 0.268085 |
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Target: 5'- cCAgugCUGCUGACCGCAucgaaaucgacaucaGUCACa-GCGCg -3' miRNA: 3'- -GUa--GGCGACUGGUGU---------------UAGUGgcCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 2160 | 0.69 | 0.387846 |
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Target: 5'- cCAUCCGCaaaaccaUGACCAgCA---GCgGGCGCa -3' miRNA: 3'- -GUAGGCG-------ACUGGU-GUuagUGgCCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 884 | 0.68 | 0.458035 |
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Target: 5'- --aCUGCUGACUucaccgGCAAaauaCAcCCGGCGCg -3' miRNA: 3'- guaGGCGACUGG------UGUUa---GU-GGCCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 445 | 0.66 | 0.555711 |
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Target: 5'- cCAgugCCGCcGACCugAuggacgcUCACCGcGUGCc -3' miRNA: 3'- -GUa--GGCGaCUGGugUu------AGUGGC-CGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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