Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25990 | 3' | -52 | NC_005340.1 | + | 19480 | 0.7 | 0.506962 |
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Target: 5'- gCCgGGGCAUCAGC---UUGACCgCCaGUu -3' miRNA: 3'- -GG-CCCGUAGUCGuuaAAUUGG-GGcCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 19535 | 0.66 | 0.748046 |
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Target: 5'- aCuGGCgGUCaAGCugau--GCCCCGGCg -3' miRNA: 3'- gGcCCG-UAG-UCGuuaaauUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20081 | 0.67 | 0.691475 |
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Target: 5'- aCGGGCAUgAcCA--UUGACgCCGGUg -3' miRNA: 3'- gGCCCGUAgUcGUuaAAUUGgGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20324 | 0.66 | 0.736942 |
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Target: 5'- gCCacaGCGUCAGCAcc---GCCCCGcGCa -3' miRNA: 3'- -GGcc-CGUAGUCGUuaaauUGGGGC-CG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20406 | 0.71 | 0.422121 |
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Target: 5'- -aGGGUAUCAgGCGc----GCCCCGGUg -3' miRNA: 3'- ggCCCGUAGU-CGUuaaauUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 23680 | 0.66 | 0.714391 |
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Target: 5'- aUGGGUAa-GGCGAgauUUUuuCCCCGGUg -3' miRNA: 3'- gGCCCGUagUCGUU---AAAuuGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 24348 | 0.7 | 0.506962 |
|
Target: 5'- cCCGuGGCGUUAGCGAgcgucuGgUCUGGCa -3' miRNA: 3'- -GGC-CCGUAGUCGUUaaau--UgGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 28873 | 0.74 | 0.288536 |
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Target: 5'- aCCGGcCAUCAgguGCAGggcuucGCCCCGGCu -3' miRNA: 3'- -GGCCcGUAGU---CGUUaaau--UGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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