Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25990 | 3' | -52 | NC_005340.1 | + | 8131 | 0.66 | 0.714391 |
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Target: 5'- uCUGGGCGUCAuuGCcg---GGCgCCGGUu -3' miRNA: 3'- -GGCCCGUAGU--CGuuaaaUUGgGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 19017 | 0.66 | 0.72572 |
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Target: 5'- -aGGGCAUCaugAGUGcUUUuGCUCCGGUc -3' miRNA: 3'- ggCCCGUAG---UCGUuAAAuUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 10031 | 0.66 | 0.730222 |
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Target: 5'- aCgGGGCAggguuaucgUCGGagaaaau-CCCCGGCa -3' miRNA: 3'- -GgCCCGU---------AGUCguuaaauuGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20324 | 0.66 | 0.736942 |
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Target: 5'- gCCacaGCGUCAGCAcc---GCCCCGcGCa -3' miRNA: 3'- -GGcc-CGUAGUCGUuaaauUGGGGC-CG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 14582 | 0.66 | 0.748046 |
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Target: 5'- gCUGaGGCugaAUCAGCcAUUgcACCgCUGGCg -3' miRNA: 3'- -GGC-CCG---UAGUCGuUAAauUGG-GGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 11671 | 0.66 | 0.748046 |
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Target: 5'- -aGGGCAUguGCcgaGAUUgaaagAACgCCGGUc -3' miRNA: 3'- ggCCCGUAguCG---UUAAa----UUGgGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 5332 | 0.66 | 0.748046 |
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Target: 5'- gCUGGcuGCAaaaguaccGCAAUgaAGCCCCGGCg -3' miRNA: 3'- -GGCC--CGUagu-----CGUUAaaUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 19535 | 0.66 | 0.748046 |
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Target: 5'- aCuGGCgGUCaAGCugau--GCCCCGGCg -3' miRNA: 3'- gGcCCG-UAG-UCGuuaaauUGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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