Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25990 | 3' | -52 | NC_005340.1 | + | 10494 | 0.69 | 0.541776 |
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Target: 5'- gCCGaccGGCGUCAGCAGaaugucgcgcauGCUCUGGCu -3' miRNA: 3'- -GGC---CCGUAGUCGUUaaau--------UGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 8638 | 0.7 | 0.506962 |
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Target: 5'- cUCGGGCAUCcGCugaccacAAUCaCCGGCg -3' miRNA: 3'- -GGCCCGUAGuCGuuaaa--UUGG-GGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 12254 | 0.7 | 0.473135 |
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Target: 5'- cCCGGcauggugGCcgCAGCAAgcuuagcGGCUCCGGCa -3' miRNA: 3'- -GGCC-------CGuaGUCGUUaaa----UUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20406 | 0.71 | 0.422121 |
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Target: 5'- -aGGGUAUCAgGCGc----GCCCCGGUg -3' miRNA: 3'- ggCCCGUAGU-CGUuaaauUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 15713 | 0.73 | 0.364392 |
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Target: 5'- aCCGuccuuGCGUacCAGCGuugUAACCCCGGCc -3' miRNA: 3'- -GGCc----CGUA--GUCGUuaaAUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 28873 | 0.74 | 0.288536 |
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Target: 5'- aCCGGcCAUCAgguGCAGggcuucGCCCCGGCu -3' miRNA: 3'- -GGCCcGUAGU---CGUUaaau--UGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 17801 | 1.14 | 0.000488 |
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Target: 5'- aCCGGGCAUCAGCAAUUUAACCCCGGCc -3' miRNA: 3'- -GGCCCGUAGUCGUUAAAUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 19535 | 0.66 | 0.748046 |
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Target: 5'- aCuGGCgGUCaAGCugau--GCCCCGGCg -3' miRNA: 3'- gGcCCG-UAG-UCGuuaaauUGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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