Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25990 | 3' | -52 | NC_005340.1 | + | 7309 | 0.71 | 0.451925 |
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Target: 5'- -aGGGCAUUGuugaaacggugcuGCAGgcUAAUCCCGGCc -3' miRNA: 3'- ggCCCGUAGU-------------CGUUaaAUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 6606 | 0.71 | 0.431245 |
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Target: 5'- gCGGGCGUCcaucaaaGGCGGUaaaAACCgCCGGUu -3' miRNA: 3'- gGCCCGUAG-------UCGUUAaa-UUGG-GGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 20406 | 0.71 | 0.422121 |
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Target: 5'- -aGGGUAUCAgGCGc----GCCCCGGUg -3' miRNA: 3'- ggCCCGUAGU-CGUuaaauUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 11460 | 0.71 | 0.421114 |
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Target: 5'- cUCGGGCGcgauauccguaaaUCAGCGAUUUAugccGCCCU-GCa -3' miRNA: 3'- -GGCCCGU-------------AGUCGUUAAAU----UGGGGcCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 15713 | 0.73 | 0.364392 |
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Target: 5'- aCCGuccuuGCGUacCAGCGuugUAACCCCGGCc -3' miRNA: 3'- -GGCc----CGUA--GUCGUuaaAUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 28873 | 0.74 | 0.288536 |
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Target: 5'- aCCGGcCAUCAgguGCAGggcuucGCCCCGGCu -3' miRNA: 3'- -GGCCcGUAGU---CGUUaaau--UGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 16207 | 0.75 | 0.27346 |
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Target: 5'- aUGGcGCggCAGCGA-UUGACCUCGGCc -3' miRNA: 3'- gGCC-CGuaGUCGUUaAAUUGGGGCCG- -5' |
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| 25990 | 3' | -52 | NC_005340.1 | + | 17801 | 1.14 | 0.000488 |
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Target: 5'- aCCGGGCAUCAGCAAUUUAACCCCGGCc -3' miRNA: 3'- -GGCCCGUAGUCGUUAAAUUGGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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