Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2622 | 3' | -58.4 | NC_001491.2 | + | 105131 | 0.66 | 0.800669 |
Target: 5'- gGGGCCgCGGCGggggucucuacGGCAGgcuuuggGGGCUCCu -3' miRNA: 3'- aCUCGGgGUCGUa----------UCGUCa------CUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 56598 | 0.66 | 0.773261 |
Target: 5'- -cGGCCCUcGCAguuuUGGUGGcGAGCUCCa -3' miRNA: 3'- acUCGGGGuCGU----AUCGUCaCUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 48832 | 0.66 | 0.773261 |
Target: 5'- cGAGgCCCAG---GGCccugGGUGGGCCuCCa -3' miRNA: 3'- aCUCgGGGUCguaUCG----UCACUCGG-GG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 4660 | 0.66 | 0.773261 |
Target: 5'- aGGGCUCCGGCccGGCGGaGAagccauCCCCg -3' miRNA: 3'- aCUCGGGGUCGuaUCGUCaCUc-----GGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 34534 | 0.66 | 0.754347 |
Target: 5'- cGGGCCCUGGgaccCAUGcCAGccgaGGGCCCCg -3' miRNA: 3'- aCUCGGGGUC----GUAUcGUCa---CUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 83292 | 0.67 | 0.748586 |
Target: 5'- --uGcCCCCAGCuggguGCGGUuuuagccacggcugcGGGCCCCg -3' miRNA: 3'- acuC-GGGGUCGuau--CGUCA---------------CUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 53527 | 0.67 | 0.744725 |
Target: 5'- gGGGCgaCCCgagAGCGcGGUAGUGAcGCCUCg -3' miRNA: 3'- aCUCG--GGG---UCGUaUCGUCACU-CGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 34015 | 0.67 | 0.741818 |
Target: 5'- -cGGUCCCAGCAgcccUcaucguccucgcccGGCGGcGAGCCCUu -3' miRNA: 3'- acUCGGGGUCGU----A--------------UCGUCaCUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 2835 | 0.67 | 0.725195 |
Target: 5'- cUGGGCCgCCGGCuccaggGGCucgGAGCgCCg -3' miRNA: 3'- -ACUCGG-GGUCGua----UCGucaCUCGgGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 70191 | 0.67 | 0.715306 |
Target: 5'- cUGAGCCCUGGCccuGCccauGUcGGCCCUg -3' miRNA: 3'- -ACUCGGGGUCGuauCGu---CAcUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 124605 | 0.67 | 0.715306 |
Target: 5'- -cGGCCugcuaauucacaCCAGCGUcGGCGGUGAGCagguauacauaCCCa -3' miRNA: 3'- acUCGG------------GGUCGUA-UCGUCACUCG-----------GGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 41163 | 0.67 | 0.705346 |
Target: 5'- -uAGCCCUAGCGuUAGCGcu-AGCCCUa -3' miRNA: 3'- acUCGGGGUCGU-AUCGUcacUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 11283 | 0.67 | 0.705346 |
Target: 5'- aGGGCgCCAGCGUA-CAGggGAGUguaCCCa -3' miRNA: 3'- aCUCGgGGUCGUAUcGUCa-CUCG---GGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 114252 | 0.68 | 0.685251 |
Target: 5'- cUGGGCCCUGGCcagcugcguGUGGCGuGUGAGCa-- -3' miRNA: 3'- -ACUCGGGGUCG---------UAUCGU-CACUCGggg -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 67572 | 0.68 | 0.675133 |
Target: 5'- cUGGGCCUgGGCGUuugGGguGaGAGCCUg -3' miRNA: 3'- -ACUCGGGgUCGUA---UCguCaCUCGGGg -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 1441 | 0.68 | 0.654802 |
Target: 5'- cGGGgCUCGGC--GGCGGgGAGCCUCg -3' miRNA: 3'- aCUCgGGGUCGuaUCGUCaCUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 9583 | 0.68 | 0.644605 |
Target: 5'- cGAGCCCCGGCugggggAGCGGU-AGCg-- -3' miRNA: 3'- aCUCGGGGUCGua----UCGUCAcUCGggg -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 31824 | 0.69 | 0.613987 |
Target: 5'- cGAGCCCCgcggagggaaGGCGaAGCGGUc-GCCCUc -3' miRNA: 3'- aCUCGGGG----------UCGUaUCGUCAcuCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 34395 | 0.69 | 0.592614 |
Target: 5'- cGAGCCCCuggagccGGCGgccCAGgccgGAGCCUCg -3' miRNA: 3'- aCUCGGGG-------UCGUaucGUCa---CUCGGGG- -5' |
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2622 | 3' | -58.4 | NC_001491.2 | + | 91044 | 0.71 | 0.504143 |
Target: 5'- cUGuAGCCUUagAGCAUGucaaaGGUGAGCCCCg -3' miRNA: 3'- -AC-UCGGGG--UCGUAUcg---UCACUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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