Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2623 | 3' | -60.7 | NC_001491.2 | + | 115429 | 0.66 | 0.675626 |
Target: 5'- -cGGGCuCCAGCGUGGauaucguugucGUGuAGUCCCg -3' miRNA: 3'- ucCCCG-GGUCGUAUCgu---------CAC-UCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 129811 | 0.66 | 0.657628 |
Target: 5'- aGGGGGUCCAGuCGUAGacauGGUucGCCUg -3' miRNA: 3'- -UCCCCGGGUC-GUAUCg---UCAcuCGGGg -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 11280 | 0.66 | 0.647598 |
Target: 5'- uAGaGGGCgCCAGCGUA-CAGggGAGUguaCCCa -3' miRNA: 3'- -UC-CCCG-GGUCGUAUcGUCa-CUCG---GGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 28550 | 0.67 | 0.617468 |
Target: 5'- cGGGGcGCCaCGGCGgggcGCAGaacGCCCCu -3' miRNA: 3'- -UCCC-CGG-GUCGUau--CGUCacuCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 69717 | 0.67 | 0.617468 |
Target: 5'- cGGcGGUugCCAGCAcgggAGUAGUGcGCUCCg -3' miRNA: 3'- uCC-CCG--GGUCGUa---UCGUCACuCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 148642 | 0.67 | 0.601426 |
Target: 5'- cGGGGCgCAGCAUacaggccggcuauucAGCGG-GGGCgcacagCCCa -3' miRNA: 3'- uCCCCGgGUCGUA---------------UCGUCaCUCG------GGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 136302 | 0.67 | 0.587434 |
Target: 5'- cGGGGCUggCGGCcuugGGCGGUGgcAGCgCCa -3' miRNA: 3'- uCCCCGG--GUCGua--UCGUCAC--UCGgGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 1605 | 0.67 | 0.587434 |
Target: 5'- cGGGGCgCAGCcaGGCcccGAGUCCCc -3' miRNA: 3'- uCCCCGgGUCGuaUCGucaCUCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 1356 | 0.67 | 0.587434 |
Target: 5'- -cGGGCuCCGGCAggcacAGCuccGUG-GCCCCc -3' miRNA: 3'- ucCCCG-GGUCGUa----UCGu--CACuCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 53526 | 0.68 | 0.577476 |
Target: 5'- uGGGGcGaCCCgagAGCGcGGUAGUGAcGCCUCg -3' miRNA: 3'- -UCCC-C-GGG---UCGUaUCGUCACU-CGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 100754 | 0.68 | 0.577476 |
Target: 5'- cGGGGCCgucccggacgCGGCGcAGCGGgGAaCCCCg -3' miRNA: 3'- uCCCCGG----------GUCGUaUCGUCaCUcGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 1067 | 0.68 | 0.557679 |
Target: 5'- cGGGGUUCAGCGUcagcggauggaGGCGG-GAGUCUa -3' miRNA: 3'- uCCCCGGGUCGUA-----------UCGUCaCUCGGGg -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 148892 | 0.68 | 0.547852 |
Target: 5'- uGGGaaaCCCAGCGaguugggaaaucUAGUGGgcUGGGCCCCa -3' miRNA: 3'- uCCCc--GGGUCGU------------AUCGUC--ACUCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 97001 | 0.68 | 0.53808 |
Target: 5'- cGGGaaGCCCGGCGUcgggagGGCcGUGGGCUaCCa -3' miRNA: 3'- uCCC--CGGGUCGUA------UCGuCACUCGG-GG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 32762 | 0.69 | 0.509147 |
Target: 5'- cGGGGCggCGGCAgcAGCAGcaucGGCCCCg -3' miRNA: 3'- uCCCCGg-GUCGUa-UCGUCac--UCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 5274 | 0.69 | 0.50629 |
Target: 5'- cGGGGCCacggaggaguggacCaucuucAGCAUcGCGGcGAGCCCCg -3' miRNA: 3'- uCCCCGG--------------G------UCGUAuCGUCaCUCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 35918 | 0.69 | 0.503438 |
Target: 5'- cGGGG-CCGGCGUguggagcuGGUGGUugagcaccccggcccGAGCCCCg -3' miRNA: 3'- uCCCCgGGUCGUA--------UCGUCA---------------CUCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 146196 | 0.69 | 0.48089 |
Target: 5'- cAGGaauuGGCCaccgAGCAUAGCGuGUGuagcGCCCCg -3' miRNA: 3'- -UCC----CCGGg---UCGUAUCGU-CACu---CGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 133270 | 0.69 | 0.47164 |
Target: 5'- gAGGGGCUCuGCGgGGCGuuuuGCCCCa -3' miRNA: 3'- -UCCCCGGGuCGUaUCGUcacuCGGGG- -5' |
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2623 | 3' | -60.7 | NC_001491.2 | + | 3580 | 0.7 | 0.462481 |
Target: 5'- uGGGGUCCAGUccggGGaCGGcgGAcGCCCCg -3' miRNA: 3'- uCCCCGGGUCGua--UC-GUCa-CU-CGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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