Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26253 | 5' | -64.3 | NC_005345.2 | + | 3027 | 0.66 | 0.23967 |
Target: 5'- cGGGCggcgGCAGcaugCUCGCuuGCGGGGg -3' miRNA: 3'- uUCCGa---CGUCcg--GAGCGggCGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 33705 | 0.66 | 0.23967 |
Target: 5'- -cGGCUcgGGGCCgUCGCggGCGAGGUg -3' miRNA: 3'- uuCCGAcgUCCGG-AGCGggCGCUCCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 15318 | 0.66 | 0.233686 |
Target: 5'- -cGGCUGUAGGUCggugCGUCgGcCGGGGc -3' miRNA: 3'- uuCCGACGUCCGGa---GCGGgC-GCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 28105 | 0.66 | 0.231915 |
Target: 5'- uGAGGC-GCAGGUgcaugggggguccuCUCGgCgGCGAGGg -3' miRNA: 3'- -UUCCGaCGUCCG--------------GAGCgGgCGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 3442 | 0.66 | 0.227827 |
Target: 5'- -cGGCgaUGCGGGCCccgaGCUCGCGcGGg -3' miRNA: 3'- uuCCG--ACGUCCGGag--CGGGCGCuCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 21374 | 0.66 | 0.227827 |
Target: 5'- -cGGcCUGCAGGCCg-GUgCGCG-GGUg -3' miRNA: 3'- uuCC-GACGUCCGGagCGgGCGCuCCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 12361 | 0.66 | 0.227827 |
Target: 5'- -cGGCUGCacgGGGCCcgaCGCCuCGCagcgGAGGg -3' miRNA: 3'- uuCCGACG---UCCGGa--GCGG-GCG----CUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 14332 | 0.66 | 0.222094 |
Target: 5'- -cGGCgagggGCAGGCgCacggCGUgCGCGAGGa -3' miRNA: 3'- uuCCGa----CGUCCG-Ga---GCGgGCGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 19133 | 0.66 | 0.222094 |
Target: 5'- -uGGCUGCGGGCCgcauggCGCgccacccggaccCCGgcCGAGGa -3' miRNA: 3'- uuCCGACGUCCGGa-----GCG------------GGC--GCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 23966 | 0.66 | 0.216483 |
Target: 5'- cGGGCUGCu-GUCgCGgCCGCGAGGc -3' miRNA: 3'- uUCCGACGucCGGaGCgGGCGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 25909 | 0.66 | 0.216483 |
Target: 5'- -cGGcCUGCgAGGCCUCGCgCGCGu--- -3' miRNA: 3'- uuCC-GACG-UCCGGAGCGgGCGCucca -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 4263 | 0.67 | 0.210995 |
Target: 5'- cGAGGCcacguUGCuGcGCCUCGCgacgcggccgCCGCGGGGc -3' miRNA: 3'- -UUCCG-----ACGuC-CGGAGCG----------GGCGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 28865 | 0.67 | 0.210995 |
Target: 5'- gGGGGCUcuucgGCGGGCacuacgggCGCCacugCGCGAGGUc -3' miRNA: 3'- -UUCCGA-----CGUCCGga------GCGG----GCGCUCCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 1207 | 0.67 | 0.205627 |
Target: 5'- -cGGCccGCGGGCCaCGCCgCGCGguacGGGUc -3' miRNA: 3'- uuCCGa-CGUCCGGaGCGG-GCGC----UCCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 26616 | 0.67 | 0.205627 |
Target: 5'- -cGGC-GCAGGCCggugUGCCCGaggacuacacCGAGGc -3' miRNA: 3'- uuCCGaCGUCCGGa---GCGGGC----------GCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 47112 | 0.68 | 0.171276 |
Target: 5'- cGGGCcGCgccGGGCC-CGCUCGaCGAGGa -3' miRNA: 3'- uUCCGaCG---UCCGGaGCGGGC-GCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 17498 | 0.68 | 0.16681 |
Target: 5'- gAAGGaaCUGCGGGCCgccggUCGCCggccggGCGAGGa -3' miRNA: 3'- -UUCC--GACGUCCGG-----AGCGGg-----CGCUCCa -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 26898 | 0.68 | 0.162448 |
Target: 5'- gAGGcGCUGCAGGCggCGCUCGcCGAccGGUa -3' miRNA: 3'- -UUC-CGACGUCCGgaGCGGGC-GCU--CCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 18631 | 0.68 | 0.15403 |
Target: 5'- cAGaGCgGCAGcacCCUCGCCCGCG-GGUc -3' miRNA: 3'- uUC-CGaCGUCc--GGAGCGGGCGCuCCA- -5' |
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26253 | 5' | -64.3 | NC_005345.2 | + | 37585 | 0.69 | 0.146011 |
Target: 5'- cGGGCUGCAGGCgggcgaccggCUCGCgUUGCGAGu- -3' miRNA: 3'- uUCCGACGUCCG----------GAGCG-GGCGCUCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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