Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26253 | 5' | -64.3 | NC_005345.2 | + | 28949 | 0.7 | 0.124189 |
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Target: 5'- --aGCUGaCGGGCCUUaUCCGCGAGGc -3' miRNA: 3'- uucCGAC-GUCCGGAGcGGGCGCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 44888 | 0.7 | 0.117614 |
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Target: 5'- cGAGGCgcaGCAacguGGCCUCGCCCGCu---- -3' miRNA: 3'- -UUCCGa--CGU----CCGGAGCGGGCGcucca -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 41097 | 0.7 | 0.114449 |
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Target: 5'- cAGGCUguuuacGCGGGCCgcagcaCGCCCGCGguacGGGg -3' miRNA: 3'- uUCCGA------CGUCCGGa-----GCGGGCGC----UCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 9689 | 0.7 | 0.111364 |
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Target: 5'- -cGGCUGCAGGCCgaucagcgggcCGCCgCGUGAGc- -3' miRNA: 3'- uuCCGACGUCCGGa----------GCGG-GCGCUCca -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 15842 | 0.72 | 0.082214 |
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Target: 5'- uGGGC-GCGGGCC-CGCUCGCGGuGGg -3' miRNA: 3'- uUCCGaCGUCCGGaGCGGGCGCU-CCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 47121 | 0.76 | 0.039529 |
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Target: 5'- -uGGCUGCAGGCCg-GCCCcggagcagcggaGCGAGGg -3' miRNA: 3'- uuCCGACGUCCGGagCGGG------------CGCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 34085 | 0.82 | 0.014083 |
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Target: 5'- cAGGcGCUGCAGGCCggCGCCCGCGAcGGc -3' miRNA: 3'- -UUC-CGACGUCCGGa-GCGGGCGCU-CCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 48694 | 1.05 | 0.000193 |
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Target: 5'- cAAGGCUGCAGGCCUCGCCCGCGAGGUg -3' miRNA: 3'- -UUCCGACGUCCGGAGCGGGCGCUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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