Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26253 | 5' | -64.3 | NC_005345.2 | + | 14332 | 0.66 | 0.222094 |
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Target: 5'- -cGGCgagggGCAGGCgCacggCGUgCGCGAGGa -3' miRNA: 3'- uuCCGa----CGUCCG-Ga---GCGgGCGCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 3442 | 0.66 | 0.227827 |
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Target: 5'- -cGGCgaUGCGGGCCccgaGCUCGCGcGGg -3' miRNA: 3'- uuCCG--ACGUCCGGag--CGGGCGCuCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 21374 | 0.66 | 0.227827 |
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Target: 5'- -cGGcCUGCAGGCCg-GUgCGCG-GGUg -3' miRNA: 3'- uuCC-GACGUCCGGagCGgGCGCuCCA- -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 12361 | 0.66 | 0.227827 |
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Target: 5'- -cGGCUGCacgGGGCCcgaCGCCuCGCagcgGAGGg -3' miRNA: 3'- uuCCGACG---UCCGGa--GCGG-GCG----CUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 28105 | 0.66 | 0.231915 |
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Target: 5'- uGAGGC-GCAGGUgcaugggggguccuCUCGgCgGCGAGGg -3' miRNA: 3'- -UUCCGaCGUCCG--------------GAGCgGgCGCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 15318 | 0.66 | 0.233686 |
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Target: 5'- -cGGCUGUAGGUCggugCGUCgGcCGGGGc -3' miRNA: 3'- uuCCGACGUCCGGa---GCGGgC-GCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 3027 | 0.66 | 0.23967 |
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Target: 5'- cGGGCggcgGCAGcaugCUCGCuuGCGGGGg -3' miRNA: 3'- uUCCGa---CGUCcg--GAGCGggCGCUCCa -5' |
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| 26253 | 5' | -64.3 | NC_005345.2 | + | 33705 | 0.66 | 0.23967 |
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Target: 5'- -cGGCUcgGGGCCgUCGCggGCGAGGUg -3' miRNA: 3'- uuCCGAcgUCCGG-AGCGggCGCUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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