Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2626 | 5' | -58.5 | NC_001491.2 | + | 105863 | 0.66 | 0.780087 |
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Target: 5'- --gGGGCAGAGgGgGAgCGGGUggaagagUGGGAg -3' miRNA: 3'- guaUCCGUUUCgCgCU-GCCCG-------GCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 69696 | 0.66 | 0.798983 |
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Target: 5'- ---uGGCGGuucccgcguccGGCGCGGCGGuugccaGCaCGGGAg -3' miRNA: 3'- guauCCGUU-----------UCGCGCUGCC------CG-GCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 22384 | 0.66 | 0.798983 |
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Target: 5'- ---cGGguGGGCGUu-CGGGCUGGGu -3' miRNA: 3'- guauCCguUUCGCGcuGCCCGGCCCu -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 44154 | 0.66 | 0.798983 |
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Target: 5'- ---cGGCGAaggacagacccAGCGCGGCGGcaGCCGcGGc -3' miRNA: 3'- guauCCGUU-----------UCGCGCUGCC--CGGC-CCu -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 28832 | 0.66 | 0.798983 |
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Target: 5'- -cUGGGcCGGGGuCGCcggagcugGGCuGGGCCGGGGu -3' miRNA: 3'- guAUCC-GUUUC-GCG--------CUG-CCCGGCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 99045 | 0.66 | 0.807758 |
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Target: 5'- aCAUAGGCcaaaauuAGCGCGGCGcGGUCugcgcgauGGAg -3' miRNA: 3'- -GUAUCCGuu-----UCGCGCUGC-CCGGc-------CCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 32187 | 0.66 | 0.816378 |
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Target: 5'- --gAGGaCGAccaGGCG-GAUGGuGCCGGGGc -3' miRNA: 3'- guaUCC-GUU---UCGCgCUGCC-CGGCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 2924 | 0.66 | 0.816378 |
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Target: 5'- --cGGGCAucGCcuccugGCuGAggUGGGCCGGGGa -3' miRNA: 3'- guaUCCGUuuCG------CG-CU--GCCCGGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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