Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2626 | 5' | -58.5 | NC_001491.2 | + | 12512 | 0.69 | 0.604153 |
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Target: 5'- --gGGGCGGGGCggcgucGCGGCGGcgGCCGuGGAc -3' miRNA: 3'- guaUCCGUUUCG------CGCUGCC--CGGC-CCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 35663 | 0.7 | 0.594097 |
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Target: 5'- ---cGGCc-GGCGCGGCgcugGGGCCGuGGAg -3' miRNA: 3'- guauCCGuuUCGCGCUG----CCCGGC-CCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 4281 | 0.7 | 0.584069 |
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Target: 5'- ---cGGCGuAGCGCGAgGcGGCCaGGAc -3' miRNA: 3'- guauCCGUuUCGCGCUgC-CCGGcCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 2447 | 0.7 | 0.574074 |
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Target: 5'- ---uGGCcgccagcuccccGAAGCGCGcgccguccCGGGCCGGGGg -3' miRNA: 3'- guauCCG------------UUUCGCGCu-------GCCCGGCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 1255 | 0.7 | 0.571084 |
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Target: 5'- gAUGGG---AGCGCGGCGGagggccugggccacGCCGGGGc -3' miRNA: 3'- gUAUCCguuUCGCGCUGCC--------------CGGCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 32882 | 0.73 | 0.397529 |
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Target: 5'- gAUGGGgAGGGUGCGAUacgggGGGaCCGGGGa -3' miRNA: 3'- gUAUCCgUUUCGCGCUG-----CCC-GGCCCU- -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 5517 | 0.83 | 0.10061 |
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Target: 5'- --cAGGUcgGGCGCGGCGGGCUGGGu -3' miRNA: 3'- guaUCCGuuUCGCGCUGCCCGGCCCu -5' |
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| 2626 | 5' | -58.5 | NC_001491.2 | + | 147032 | 1.09 | 0.001681 |
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Target: 5'- aCAUAGGCAAAGCGCGACGGGCCGGGAa -3' miRNA: 3'- -GUAUCCGUUUCGCGCUGCCCGGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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