Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 26270 | 5' | -58 | NC_005345.2 | + | 43114 | 0.7 | 0.280294 |
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Target: 5'- cGGUgagcuGCAGUGGCUGCUCGUcguacacGGUGg -3' miRNA: 3'- uCCGacu--UGUCGCCGACGGGCA-------UCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 18793 | 0.71 | 0.238951 |
|
Target: 5'- cGGGCUGccgucgggcaggguGACGGCGGCgcggGCCUgGUAGUc -3' miRNA: 3'- -UCCGAC--------------UUGUCGCCGa---CGGG-CAUCAc -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 47715 | 0.71 | 0.260286 |
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Target: 5'- aGGGCgaaGAGCuGCGGCUGCCagcuGUGc -3' miRNA: 3'- -UCCGa--CUUGuCGCCGACGGgcauCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 20255 | 0.77 | 0.100071 |
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Target: 5'- gAGGCaUGGacgaccucgcGCAGUGGC-GCCCGUAGUGc -3' miRNA: 3'- -UCCG-ACU----------UGUCGCCGaCGGGCAUCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 39766 | 1.09 | 0.000408 |
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Target: 5'- cAGGCUGAACAGCGGCUGCCCGUAGUGg -3' miRNA: 3'- -UCCGACUUGUCGCCGACGGGCAUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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