miRNA: 3'- uCCG----ACU---UGUCGCCGaCGGGC-AUCAc -5'
miRNA: 3'- uCCGacu--UGUCGCCGACGGGCA-------UCAC- -5'
miRNA: 3'- uCCGACuug-UCGCCGACGGGca--UCAC- -5'
miRNA: 3'- -UCCGa--CUUGuCGCCGACGGgcauCAC- -5'
miRNA: 3'- uCCGacuUGUCGCCGaCGGGca---UCAC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26270 | 5' | -58 | NC_005345.2 | + | 37425 | 0.69 | 0.350938 |
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Target: 5'- cGGCgaguUGGuacGCGGCGGCgGCCCGcUGGUc -3' miRNA: 3'- uCCG----ACU---UGUCGCCGaCGGGC-AUCAc -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 43114 | 0.7 | 0.280294 |
|
Target: 5'- cGGUgagcuGCAGUGGCUGCUCGUcguacacGGUGg -3' miRNA: 3'- uCCGacu--UGUCGCCGACGGGCA-------UCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 4918 | 0.7 | 0.273957 |
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Target: 5'- cGGCUGcguaGGCGGUUGCCCacacGGUGg -3' miRNA: 3'- uCCGACuug-UCGCCGACGGGca--UCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 47715 | 0.71 | 0.260286 |
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Target: 5'- aGGGCgaaGAGCuGCGGCUGCCagcuGUGc -3' miRNA: 3'- -UCCGa--CUUGuCGCCGACGGgcauCAC- -5' |
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| 26270 | 5' | -58 | NC_005345.2 | + | 34151 | 0.66 | 0.523809 |
|
Target: 5'- uGGC---GCGGCGGCgGCCCcgaggAGUGg -3' miRNA: 3'- uCCGacuUGUCGCCGaCGGGca---UCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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