miRNA: 3'- cCCGCUaUGa-----GUGGACAGGa---CGCugC- -5'
miRNA: 3'- -CCCGCUa---------UGAGugGacaGGaCGCUGC- -5'
miRNA: 3'- cCCGCUA------UGAGUGGa-CAGGaCGcuGC- -5'
miRNA: 3'- ccC-GCUAUGAGUGGac--AgGACGCUGc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26326 | 5' | -55.2 | NC_005345.2 | + | 35284 | 0.66 | 0.716673 |
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Target: 5'- cGGCGGaGCgagcugUACUUGUCCgcgaGCGugGg -3' miRNA: 3'- cCCGCUaUGa-----GUGGACAGGa---CGCugC- -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 30520 | 0.66 | 0.727325 |
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Target: 5'- cGGGCGAgggugcugccGCUCugCgugCCgGUGACGg -3' miRNA: 3'- -CCCGCUa---------UGAGugGacaGGaCGCUGC- -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 12237 | 0.66 | 0.727325 |
|
Target: 5'- uGGCGAUgaucccGCUCACCcgGUCCcGCuuCGu -3' miRNA: 3'- cCCGCUA------UGAGUGGa-CAGGaCGcuGC- -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 4899 | 0.66 | 0.727325 |
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Target: 5'- -aGuCGAUGCUCGCCgagcUgCUGCGGCa -3' miRNA: 3'- ccC-GCUAUGAGUGGac--AgGACGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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