Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26326 | 5' | -55.2 | NC_005345.2 | + | 15509 | 0.69 | 0.552895 |
Target: 5'- cGGGCGuUGCUC--UUGUCCUGCucgaucgccugGACGa -3' miRNA: 3'- -CCCGCuAUGAGugGACAGGACG-----------CUGC- -5' |
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26326 | 5' | -55.2 | NC_005345.2 | + | 35876 | 0.71 | 0.393548 |
Target: 5'- gGGGCGucgucgccgacgagcUGCUCGCCgGUCgUGcCGACGg -3' miRNA: 3'- -CCCGCu--------------AUGAGUGGaCAGgAC-GCUGC- -5' |
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26326 | 5' | -55.2 | NC_005345.2 | + | 2160 | 1.12 | 0.000629 |
Target: 5'- cGGGCGAUACUCACCUGUCCUGCGACGa -3' miRNA: 3'- -CCCGCUAUGAGUGGACAGGACGCUGC- -5' |
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26326 | 5' | -55.2 | NC_005345.2 | + | 30520 | 0.66 | 0.727325 |
Target: 5'- cGGGCGAgggugcugccGCUCugCgugCCgGUGACGg -3' miRNA: 3'- -CCCGCUa---------UGAGugGacaGGaCGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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