miRNA: 3'- -CCCGCU-AUGAGUGGaCAGgaCGCUGC- -5'
miRNA: 3'- -CCCGCuaUGa---------G--------------UGGACAGGACGCUGc -5'
miRNA: 3'- -CCCGCU-------AUGAGUGGA-------CAGGACgCUGC- -5'
miRNA: 3'- -CCCGCUAUGAGUGGACAGGACGCUGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26326 | 5' | -55.2 | NC_005345.2 | + | 16558 | 0.72 | 0.383644 |
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Target: 5'- cGGGUGGcUGCUCACC-GUC--GCGACGa -3' miRNA: 3'- -CCCGCU-AUGAGUGGaCAGgaCGCUGC- -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 10673 | 0.74 | 0.291818 |
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Target: 5'- gGGGCGcgGCgcgggaagugCccgcgcugugcccgGCCUGUCCUGCGGCa -3' miRNA: 3'- -CCCGCuaUGa---------G--------------UGGACAGGACGCUGc -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 43080 | 0.74 | 0.264376 |
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Target: 5'- gGGGUGGcccgagcUGCUCGCCUggauccgGUCCUGgGGCGu -3' miRNA: 3'- -CCCGCU-------AUGAGUGGA-------CAGGACgCUGC- -5' |
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| 26326 | 5' | -55.2 | NC_005345.2 | + | 2160 | 1.12 | 0.000629 |
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Target: 5'- cGGGCGAUACUCACCUGUCCUGCGACGa -3' miRNA: 3'- -CCCGCUAUGAGUGGACAGGACGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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