Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26427 | 3' | -55.1 | NC_005345.2 | + | 4124 | 0.66 | 0.688616 |
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Target: 5'- gGUUGGCGuucgagcggcugcucGcGGCGCUCGUcGAGCGGg- -3' miRNA: 3'- gCAGCCGC---------------U-CUGUGAGCA-CUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 10074 | 0.66 | 0.695131 |
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Target: 5'- aCGcCGuCGAGGCGCUCGUcauccgggccGAGCGGc- -3' miRNA: 3'- -GCaGCcGCUCUGUGAGCA----------CUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 19895 | 0.66 | 0.695131 |
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Target: 5'- uGUUgcgGGCGAGccgcuGCGCaUCGcGGGCGAUGa -3' miRNA: 3'- gCAG---CCGCUC-----UGUG-AGCaCUCGCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 38409 | 0.66 | 0.705938 |
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Target: 5'- gCGUCGGCGAcGACAUc---GAGCGcgGc -3' miRNA: 3'- -GCAGCCGCU-CUGUGagcaCUCGCuaC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 7325 | 0.66 | 0.716674 |
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Target: 5'- aGUCGGCG-GACGCcgagUCGcacGCGGUGu -3' miRNA: 3'- gCAGCCGCuCUGUG----AGCacuCGCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 37337 | 0.66 | 0.716674 |
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Target: 5'- --aCGGCGAuGACccguCUCG-GAGCGAg- -3' miRNA: 3'- gcaGCCGCU-CUGu---GAGCaCUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 13267 | 0.66 | 0.737882 |
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Target: 5'- --cCGGCGAGcaGCUCGUcGGCGAc- -3' miRNA: 3'- gcaGCCGCUCugUGAGCAcUCGCUac -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 1334 | 0.66 | 0.737882 |
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Target: 5'- gGUCcuccagGGCGAGGCGCUgucgaaGUGGGuUGAUGu -3' miRNA: 3'- gCAG------CCGCUCUGUGAg-----CACUC-GCUAC- -5' |
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| 26427 | 3' | -55.1 | NC_005345.2 | + | 22249 | 0.66 | 0.737882 |
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Target: 5'- uCGaCGGCGAGGUGCUgcCGUGGGCGc-- -3' miRNA: 3'- -GCaGCCGCUCUGUGA--GCACUCGCuac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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