Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26430 | 3' | -62.8 | NC_005345.2 | + | 30140 | 0.72 | 0.122786 |
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Target: 5'- aGGGCGGcgcgccacccgucgaGGUCGUCcCGCCGGCCa- -3' miRNA: 3'- -CUCGCCag-------------CCAGCAGaGCGGCCGGgu -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 35867 | 0.66 | 0.338744 |
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Target: 5'- cGGgGGUCGGgggCGUCgUCGCCGacgagcuGCUCGc -3' miRNA: 3'- cUCgCCAGCCa--GCAG-AGCGGC-------CGGGU- -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 37853 | 0.69 | 0.193349 |
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Target: 5'- -uGCGGUCGGUCG-Cg-GCCGGCg-- -3' miRNA: 3'- cuCGCCAGCCAGCaGagCGGCCGggu -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 40063 | 0.68 | 0.248739 |
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Target: 5'- -uGCGGgCGGUCGcCggcgaCGgCGGCCCGg -3' miRNA: 3'- cuCGCCaGCCAGCaGa----GCgGCCGGGU- -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 43056 | 0.66 | 0.338744 |
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Target: 5'- -cGCGGugccggcUCGGUCGUgUCGUCGGgCa- -3' miRNA: 3'- cuCGCC-------AGCCAGCAgAGCGGCCgGgu -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 43920 | 0.67 | 0.281053 |
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Target: 5'- cAGCGcGUCGG-CGaUCagGCCGGCCgCGa -3' miRNA: 3'- cUCGC-CAGCCaGC-AGagCGGCCGG-GU- -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 45904 | 0.69 | 0.214083 |
|
Target: 5'- cGGCGGaCGGUUGUCacUCGCCuGCCa- -3' miRNA: 3'- cUCGCCaGCCAGCAG--AGCGGcCGGgu -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 46407 | 0.66 | 0.316613 |
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Target: 5'- aGGCGGcacuugUCGaccGcCGUCUCGCCGcGUCCAg -3' miRNA: 3'- cUCGCC------AGC---CaGCAGAGCGGC-CGGGU- -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 47437 | 1.08 | 0.000218 |
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Target: 5'- cGAGCGGUCGGUCGUCUCGCCGGCCCAa -3' miRNA: 3'- -CUCGCCAGCCAGCAGAGCGGCCGGGU- -5' |
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| 26430 | 3' | -62.8 | NC_005345.2 | + | 48363 | 0.68 | 0.254947 |
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Target: 5'- aAGCGGgccCGGUCGgcaucccccgC-CGCCGGCCgGg -3' miRNA: 3'- cUCGCCa--GCCAGCa---------GaGCGGCCGGgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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