Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26443 | 3' | -54 | NC_005357.1 | + | 4391 | 0.66 | 0.749036 |
Target: 5'- --aGGCGccGUcGCGCCGGGCCuugagcGUGCGg -3' miRNA: 3'- cgaUUGC--CAaUGUGGCCCGGc-----UACGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 31942 | 0.66 | 0.749036 |
Target: 5'- aGC--ACGGcccguUCGGGCCGGUGCu -3' miRNA: 3'- -CGauUGCCaauguGGCCCGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 15970 | 0.66 | 0.738384 |
Target: 5'- aCUGACGG--AUGCCGaGGUCGA-GCGc -3' miRNA: 3'- cGAUUGCCaaUGUGGC-CCGGCUaCGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 776 | 0.66 | 0.727621 |
Target: 5'- cUUGcCGGUcAgAUCGGGCCGcUGCGc -3' miRNA: 3'- cGAUuGCCAaUgUGGCCCGGCuACGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 39254 | 0.66 | 0.727621 |
Target: 5'- cGC-AGCGG--ACGCCGGGCUugccAUGCu -3' miRNA: 3'- -CGaUUGCCaaUGUGGCCCGGc---UACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 5932 | 0.66 | 0.716759 |
Target: 5'- cGCUGGCGGcaucccagacgUACACgGGGCCauUGUc -3' miRNA: 3'- -CGAUUGCCa----------AUGUGgCCCGGcuACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 12454 | 0.66 | 0.705809 |
Target: 5'- --cGACGGcUGCAuCCaGGCCGucGUGCGc -3' miRNA: 3'- cgaUUGCCaAUGU-GGcCCGGC--UACGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 17347 | 0.67 | 0.694785 |
Target: 5'- cGCcgGGCGcGcUGCGCUGGGUggCGAUGCu -3' miRNA: 3'- -CGa-UUGC-CaAUGUGGCCCG--GCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 29598 | 0.67 | 0.693679 |
Target: 5'- gGCgcGCGGUUugccaguuguccaGCGCCGGGCgcuUGUGg -3' miRNA: 3'- -CGauUGCCAA-------------UGUGGCCCGgcuACGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 15142 | 0.67 | 0.683697 |
Target: 5'- uGCUGGCGGcggaUGCGCCcagggcgacGGCUGAUGUu -3' miRNA: 3'- -CGAUUGCCa---AUGUGGc--------CCGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 6663 | 0.67 | 0.683697 |
Target: 5'- cGCUcAUGGUcUGCGCCacGCCGAUGUu -3' miRNA: 3'- -CGAuUGCCA-AUGUGGccCGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 37040 | 0.67 | 0.680361 |
Target: 5'- uGCUcGCGGcgcGCACCagugacauaacccuGGGCgCGGUGCa -3' miRNA: 3'- -CGAuUGCCaa-UGUGG--------------CCCG-GCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 4628 | 0.67 | 0.672559 |
Target: 5'- --cGGCGGggAUGCUGGGCCacgcUGCGu -3' miRNA: 3'- cgaUUGCCaaUGUGGCCCGGcu--ACGC- -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 23290 | 0.67 | 0.672559 |
Target: 5'- gGCUGACGGUgugg--GGGCCGAacacccUGCa -3' miRNA: 3'- -CGAUUGCCAauguggCCCGGCU------ACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 40338 | 0.67 | 0.66138 |
Target: 5'- ---cACGGcgAcCACCuGGGCCGAUGUc -3' miRNA: 3'- cgauUGCCaaU-GUGG-CCCGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 15590 | 0.67 | 0.637826 |
Target: 5'- cGCU-GCGGaUGCACCGcgccaucGGCCagGAUGCu -3' miRNA: 3'- -CGAuUGCCaAUGUGGC-------CCGG--CUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 5388 | 0.68 | 0.627719 |
Target: 5'- uGCUGACGGUgcuCACgucGCCGAUGUu -3' miRNA: 3'- -CGAUUGCCAau-GUGgccCGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 34648 | 0.68 | 0.60528 |
Target: 5'- gGCgAAgGGUggACGCCGGcgcagaucgcGCCGGUGCu -3' miRNA: 3'- -CGaUUgCCAa-UGUGGCC----------CGGCUACGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 33689 | 0.68 | 0.60528 |
Target: 5'- uGUUGACGGUaGCGCCGcgcccGCCGG-GCa -3' miRNA: 3'- -CGAUUGCCAaUGUGGCc----CGGCUaCGc -5' |
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26443 | 3' | -54 | NC_005357.1 | + | 8604 | 0.68 | 0.60528 |
Target: 5'- uGCUGGauaagcUGGUUGCGCUucucGGCCGcUGCGg -3' miRNA: 3'- -CGAUU------GCCAAUGUGGc---CCGGCuACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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