Results 1 - 20 of 58 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26447 | 3' | -58 | NC_005357.1 | + | 744 | 0.66 | 0.438668 |
Target: 5'- cCGGCCggCGCCGccguaggcgGCCUGgUGGCUa-- -3' miRNA: 3'- uGCUGGa-GCGGU---------UGGACgACCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 13034 | 0.66 | 0.437688 |
Target: 5'- gACGGCCUCGUCAucGCgUUGCUugagugcGGCCg-- -3' miRNA: 3'- -UGCUGGAGCGGU--UG-GACGA-------CCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 1779 | 0.66 | 0.468629 |
Target: 5'- gACGcCCUCGCCGGCaC-GCcGGCCc-- -3' miRNA: 3'- -UGCuGGAGCGGUUG-GaCGaCCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 6587 | 0.66 | 0.448535 |
Target: 5'- gACGACCccgaaaaacUCGCCAGaaaCUGCUcGCCa-- -3' miRNA: 3'- -UGCUGG---------AGCGGUUg--GACGAcCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 38337 | 0.66 | 0.438668 |
Target: 5'- aGCGccgcccACCUgGCCGGCCUGaUGGCUa-- -3' miRNA: 3'- -UGC------UGGAgCGGUUGGACgACCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 2925 | 0.66 | 0.489175 |
Target: 5'- cAUGGCUcUGCCAACgCUacggcuuggcggGCUGGCCUUu -3' miRNA: 3'- -UGCUGGaGCGGUUG-GA------------CGACCGGAAc -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 28547 | 0.66 | 0.468629 |
Target: 5'- cACGACCUUG-CGGCC-GCcGGCCa-- -3' miRNA: 3'- -UGCUGGAGCgGUUGGaCGaCCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 34465 | 0.66 | 0.468629 |
Target: 5'- gACGAacucauCgUUGCCGACCUGaaauaUGGCCgUGg -3' miRNA: 3'- -UGCU------GgAGCGGUUGGACg----ACCGGaAC- -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 13443 | 0.66 | 0.468629 |
Target: 5'- -aGGCC-CGCCAGCUUGgC-GGCCUc- -3' miRNA: 3'- ugCUGGaGCGGUUGGAC-GaCCGGAac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 37181 | 0.66 | 0.448535 |
Target: 5'- cCGACC---CCGACCUGCcGGCCg-- -3' miRNA: 3'- uGCUGGagcGGUUGGACGaCCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 5570 | 0.66 | 0.448535 |
Target: 5'- uCGACggCGCCAAC--GCUGGCCg-- -3' miRNA: 3'- uGCUGgaGCGGUUGgaCGACCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 18354 | 0.66 | 0.438668 |
Target: 5'- gAUGACCaccuguUUGCCGGCggcgagGUUGGCCUUGc -3' miRNA: 3'- -UGCUGG------AGCGGUUGga----CGACCGGAAC- -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 13701 | 0.67 | 0.391277 |
Target: 5'- gACGGCCgCGCCgGACUUGCUG-CCg-- -3' miRNA: 3'- -UGCUGGaGCGG-UUGGACGACcGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 24010 | 0.67 | 0.428926 |
Target: 5'- cCGACCUCGCCGgGCgUGC--GCCUg- -3' miRNA: 3'- uGCUGGAGCGGU-UGgACGacCGGAac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 32988 | 0.67 | 0.382207 |
Target: 5'- cUGGCCgaacaGCCAGCCgUGCaGGCCgUGg -3' miRNA: 3'- uGCUGGag---CGGUUGG-ACGaCCGGaAC- -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 13083 | 0.67 | 0.382207 |
Target: 5'- -aGGCCgUCGCCAcuGCgCUGCgcGGCCUg- -3' miRNA: 3'- ugCUGG-AGCGGU--UG-GACGa-CCGGAac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 35749 | 0.67 | 0.413609 |
Target: 5'- uCGGCCUuggCGCCccacuuuuccuuggcGACCUGCucgaUGGCCUg- -3' miRNA: 3'- uGCUGGA---GCGG---------------UUGGACG----ACCGGAac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 28877 | 0.67 | 0.391277 |
Target: 5'- cACGuCCUCGCUgcuGGCguagUUGCUGGCCg-- -3' miRNA: 3'- -UGCuGGAGCGG---UUG----GACGACCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 11366 | 0.67 | 0.404208 |
Target: 5'- gGCGACCugcUCGCCcggcaccucgguggcGACCUGaugGGCCa-- -3' miRNA: 3'- -UGCUGG---AGCGG---------------UUGGACga-CCGGaac -5' |
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26447 | 3' | -58 | NC_005357.1 | + | 24748 | 0.67 | 0.416456 |
Target: 5'- cGCGGCCUCGaCCcaugcugucggaauGACCUGCcacgGGUCg-- -3' miRNA: 3'- -UGCUGGAGC-GG--------------UUGGACGa---CCGGaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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