Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26452 | 3' | -65.2 | NC_005357.1 | + | 14203 | 0.66 | 0.208895 |
Target: 5'- uCCGACCCgcgcGUGGUcgucGGCGU-GGCCGAc -3' miRNA: 3'- cGGCUGGGa---CGCCG----UCGCGcCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 17635 | 0.66 | 0.20359 |
Target: 5'- uGCCGcAUCgUGCGGCAGUaC-GGCCAc -3' miRNA: 3'- -CGGC-UGGgACGCCGUCGcGcCCGGUu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 36056 | 0.66 | 0.20359 |
Target: 5'- gGCCGACCUgGUGuaaccCAGCGCcccggccuucGGGCCGGg -3' miRNA: 3'- -CGGCUGGGaCGCc----GUCGCG----------CCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 34317 | 0.66 | 0.20359 |
Target: 5'- cGCCGGCC--GC-GCGGUGCaGGGCCu- -3' miRNA: 3'- -CGGCUGGgaCGcCGUCGCG-CCCGGuu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 38399 | 0.66 | 0.20359 |
Target: 5'- cGCuCGACCCgcGCGGCAuGaCGCugguGGCCGg -3' miRNA: 3'- -CG-GCUGGGa-CGCCGU-C-GCGc---CCGGUu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 36045 | 0.66 | 0.203066 |
Target: 5'- gGCCGGCcggCCUGCGccugauuGCcGaCGUGGGCCGGu -3' miRNA: 3'- -CGGCUG---GGACGC-------CGuC-GCGCCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 8852 | 0.66 | 0.203066 |
Target: 5'- cGCCGacguaGCCCaggacggUGCGGUAGCGUucGGCCu- -3' miRNA: 3'- -CGGC-----UGGG-------ACGCCGUCGCGc-CCGGuu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 24234 | 0.66 | 0.200463 |
Target: 5'- gGCCGAUCUUcucgaugauguccucGCGGCuugccaccuugAGCGUGGcGCCGGa -3' miRNA: 3'- -CGGCUGGGA---------------CGCCG-----------UCGCGCC-CGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 13312 | 0.66 | 0.193329 |
Target: 5'- gGCCGGCgUUGUaGGCGGCGUaGGCUu- -3' miRNA: 3'- -CGGCUGgGACG-CCGUCGCGcCCGGuu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 37834 | 0.66 | 0.192827 |
Target: 5'- gGCUGACCUggGCGGCAuCGCauaacuaGGaGCCAAc -3' miRNA: 3'- -CGGCUGGGa-CGCCGUcGCG-------CC-CGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 11379 | 0.66 | 0.18837 |
Target: 5'- cCCGGCaCCU-CGGUGGCGaccugauGGGCCAGg -3' miRNA: 3'- cGGCUG-GGAcGCCGUCGCg------CCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 2446 | 0.66 | 0.18837 |
Target: 5'- cGCCGAaaCUGCGGCccaGGCGCucGCCu- -3' miRNA: 3'- -CGGCUggGACGCCG---UCGCGccCGGuu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 9811 | 0.66 | 0.18837 |
Target: 5'- -gCGACCgCggcgucGCGGCccuuGaCGCGGGCCAGc -3' miRNA: 3'- cgGCUGG-Ga-----CGCCGu---C-GCGCCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 14601 | 0.66 | 0.18837 |
Target: 5'- gGCCGGCgCCaccGCgGGCAGCGCGcGCgAGg -3' miRNA: 3'- -CGGCUG-GGa--CG-CCGUCGCGCcCGgUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 25116 | 0.66 | 0.185448 |
Target: 5'- aGCCGGCCCauaugccggaauggaUGCGcGCc-CGCGGcGCCGg -3' miRNA: 3'- -CGGCUGGG---------------ACGC-CGucGCGCC-CGGUu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 16902 | 0.67 | 0.180668 |
Target: 5'- cGCUG-CCg-GCGGCGGCaaaaccgaccucaucGCGGGCCu- -3' miRNA: 3'- -CGGCuGGgaCGCCGUCG---------------CGCCCGGuu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 39489 | 0.67 | 0.178787 |
Target: 5'- -aCGugCCgcGCGacGCGGUGUGGGCCGg -3' miRNA: 3'- cgGCugGGa-CGC--CGUCGCGCCCGGUu -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 30414 | 0.67 | 0.17416 |
Target: 5'- cGCCGGCCa--CGGCcGCGCugcuGGCCGAc -3' miRNA: 3'- -CGGCUGGgacGCCGuCGCGc---CCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 8831 | 0.67 | 0.17416 |
Target: 5'- gGUCGGgCCaGCcGCAaCGCGGGCCAGc -3' miRNA: 3'- -CGGCUgGGaCGcCGUcGCGCCCGGUU- -5' |
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26452 | 3' | -65.2 | NC_005357.1 | + | 41428 | 0.67 | 0.17416 |
Target: 5'- cCCGGCCa-GUGGCgcgGGCaGCGGGCUGAc -3' miRNA: 3'- cGGCUGGgaCGCCG---UCG-CGCCCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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